LOCUS       pNLex(NLS)  10174 bp    DNA   circular             5-SEP-2003
SOURCE      
  ORGANISM  
COMMENT     Encodes LexA-NLS.  Predicted pI = 8.99, MW=26,754.8
            
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|200319280|
COMMENT     VNTDBDATE|312926264|
COMMENT     VNTNAME|pNLex(NLS)|
COMMENT     VNTAUTHORNAME|Finley|
FEATURES             Location/Qualifiers
     promoter        67..1511
                     /vntifkey="29"
                     /label=ADH1\promoter
                     /note="ADH1 promoter"
     CDS             1538..2143
                     /vntifkey="4"
                     /label=LexA
                     /note="LexA DBD (amino acids 1-202)"
     primer_bind     2105..2143
                     /vntifkey="28"
                     /label=5'\RF126
                     /note="homologous to 5' end of RF126 primer"
     primer_bind     2126..2143
                     /vntifkey="28"
                     /label=3'\3KF-BD
                     /note="homologous to 3' end of 3KF-BD primer"
     primer          1776..1796
                     /vntifkey="27"
                     /label=LexA\Central-s
                     /note="LexA CDS primer, located in centre of DBD ORF"
     primer_bind     1541..1565
                     /vntifkey="28"
                     /label=3'\RFx129
                     /note="priming site for the 3' end of RFx129. Amplifies all but ATG of LexA"
     primer          2117..2143
                     /vntifkey="27"
                     /label=LexA\3'
                     /note="sense primer for PCR amplification of LexA DBD plasmid inserts"
     primer          2119..2139
                     /vntifkey="27"
                     /label=JZ45
                     /note="hybridize to the very end of LexA"
     primer_bind     1878..1902
                     /vntifkey="28"
                     /label=LexA-3PR
                     /note="primer for nested PCR in LexA-CS products, also for sequencing"
     CDS             2150..2173
                     /vntifkey="4"
                     /label=NLS
                     /note="Nuclear Localisation Sequence"
     CDS             complement(6122..6781)
                     /vntifkey="4"
                     /label=HIS3
                     /note="HIS3 gene, location according to Genbank file"
     CDS             8139..8999
                     /vntifkey="4"
                     /label=ampR
                     /note="ampicillin  resistance gene"
     rep_origin      <2898..>4794
                     /vntifkey="33"
                     /label=2\µm\origin
                     /note="2µm origin, the part that matches part of YEP24"
     terminator      2217..2547
                     /vntifkey="43"
                     /label=ADH\T
                     /note="ADH Terminator region"
     primer_bind     complement(2272..2296)
                     /vntifkey="28"
                     /label=JF16
                     /note="anti-sense ADH T primer"
     primer          complement(2385..2402)
                     /vntifkey="27"
                     /label=CAS_02
                     /note="anti-sense ADH T primer, flanking JF16, works well with lexA Central-s"
     primer_bind     complement(2213..2230)
                     /vntifkey="28"
                     /label=pEG202R
                     /note="reverse primer for ADH terminator region"
     old_sequence    <9011..>49
                     /vntifkey="89"
                     /label=pBr\ori
                     /note="pBR backbone and ori"
BASE COUNT     2900 a      2241 c      2277 g      2756 t 
ORIGIN
        1 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggatgatccg 
       61 ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg caaaagacaa 
      121 atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt tggctttgcg 
      181 gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg cggacccgcg 
      241 ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag ttttttgcgc 
      301 ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca ataagaatgc 
      361 cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa gttgccgaaa 
      421 gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac ttgcgagacg 
      481 cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga gacgcgcata 
      541 accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat aaatagacag 
      601 gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg 
      661 cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca catatattaa 
      721 ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt ttccgatttt 
      781 tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta caatatggac 
      841 ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct tcgttggtct 
      901 ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag acaagacata 
      961 atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac ataacgaact 
     1021 aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta ccctttttcc 
     1081 atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt tttttctttt 
     1141 ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac 
     1201 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg 
     1261 aggggtatct tcgaacacac gaaacttttt ccttccttca ttcacgcaca ctactctcta 
     1321 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaagtttgc 
     1381 cgctttgcta tcaagtataa atagacctgc aattattaat cttttgtttc ctcgtcattg 
     1441 ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct ataccaagca 
     1501 tacaatcaac tccaagcttg aattaattcc gggcggaatg aaagcgttaa cggccaggca 
     1561 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg 
     1621 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa 
     1681 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct 
     1741 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccact 
     1801 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttat tcaagccgaa 
     1861 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg 
     1921 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg 
     1981 tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt 
     2041 gccagaaaat agcgagttta aaccaattgt cgtagatctt cgtcagcaga gcttcaccat 
     2101 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctggaattgg cccccaagaa 
     2161 aaagagaaag gtggaattcc cggggatccg tcgacctgca gccaagctaa ttccgggcga 
     2221 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca 
     2281 aattttaaag tgactcttag gttttaaaac gaaaattctt gttcttgagt aactctttcc 
     2341 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta 
     2401 ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cacccaattg tagatatgct 
     2461 aactccagca atgagttgat gaatctcggt gtgtatttta tgtcctcaga ggacaacacc 
     2521 tgttgtaatc gttcttccac acggatcgat ccacaggacg ggtgtggtcg ccatgatcgc 
     2581 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc 
     2641 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag 
     2701 cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag 
     2761 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat 
     2821 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa 
     2881 actaccgcat taaagctagc tttgaagaaa aatgcgcctt attcaatctt tgctataaaa 
     2941 aatggcccaa aatctcacat tggaagacat ttgatgacct catttctttc aatgaagggc 
     3001 ctaacggagt tgactaatgt tgtgggaaat tggagcgata agcgtgcttc tgccgtggcc 
     3061 aggacaacgt atactcatca gataacagca atacctgatc actacttcgc actagtttct 
     3121 cggtactatg catatgatcc aatatcaaag gaaatgatag cattgaagga tgagactaat 
     3181 ccaattgagg agtggcagca tatagaacag ctaaagggta gtgctgaagg aagcatacga 
     3241 taccccgcat ggaatgggat aatatcacag gaggtactag actacctttc atcctacata 
     3301 aatagacgca tataagtacg catttaagca taaacacgca ctatgccgtt cttctcatgt 
     3361 atatatatat acaggcaaca cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc 
     3421 gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa acgctttgaa gttcctattc 
     3481 cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg 
     3541 ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat 
     3601 attgcgaata ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt 
     3661 gctatatccc tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc 
     3721 gacctctaca ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta 
     3781 agaactattc atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag 
     3841 tatatagaga caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa 
     3901 cgctatcact ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg 
     3961 gatgccttta tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa 
     4021 gtggagtcag gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc 
     4081 ttaacggacc tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag 
     4141 aaaaaaagta atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta 
     4201 gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg 
     4261 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt 
     4321 ttgttttaca aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat 
     4381 ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg 
     4441 catttttgtt ctacaaaatg aagcacagat gcttcgttaa caaagatatg ctattgaagt 
     4501 gcaagatgga aacgcagaaa atgaaccggg gatgcgacgt gcaagattac ctatgcaata 
     4561 gatgcaatag tttctccagg aaccgaaata catacattgt cttccgtaaa gcgctagact 
     4621 atatattatt atacaggttc aaatatacta tctgtttcag ggaaaactcc caggttcgga 
     4681 tgttcaaaat tcaatgatgg gtaacaagta cgatcgtaaa tctgtaaaac agtttgtcgg 
     4741 atattaggct gtatctcctc aaagcgtatt cgaatatcat tgagaagctg cagcaggcgt 
     4801 gaagttagac gacaacttct ctctggaaac gcataccgat attcaggctg ctgcaaaggc 
     4861 acaggctagt gcccgtgcga gtgcatccgg taccacccca gatgctgtag tagcttctgg 
     4921 tagcactgca atgagccatg cttatcaaga aaacacaggt tttggtactc gtcccatata 
     4981 tcttgacatg caagccacta caccaacaga ccctagggtt ttggatacga tgttgaagtt 
     5041 ttatacggga ctttatggta atcctcattc caacactcac tcttacggtt gggaaacaaa 
     5101 tactgctgtg gaaaatgcta gagctcacgt agcaaagatg atcaatgccg accccaagga 
     5161 aataatattc acttcgggag cgaccgaatc taataatatg gttcttaagg gtgtcccaag 
     5221 attttataag aagactaaga aacacatcat caccactaga acggaacaca agtgtgtctt 
     5281 ggaagccgca cgggccatga tgaaggaggg atttgaagtc actttcctaa atgtggacga 
     5341 tcaaggtctt atcgatttga aggaattgga agatgccatt agaccagata cctgtctcgt 
     5401 ctctgtgatg gctgtcaata atgaaatcgg tgtcattcaa cctattaaag aaattggagc 
     5461 aatttgtaga aagaataaga tcctcgggga caccaaatat ggcgatctcg gccttttcgt 
     5521 ttcttggagc tgggacatgt ttgccatcga tccatctacc accagaacgg ccgttagatc 
     5581 tgctgccacc gttgtttcca ccgaagaaac caccgttgcc gtaaccacca cgacggttgt 
     5641 tgctaaagaa gctgccaccg ccacggccac cgttgtagcc gccgttgttg ttattgtagt 
     5701 tgctactgtt atttctggca cttcttggtt ttcctcttaa gtgaggagga acataaccat 
     5761 tctcgttgtt gtcgttgatg cttaaatttt gcacttgttc gctcagttca gccataatat 
     5821 gaaatgcttt tcttgttgtt cttacggaat accacttgcc acctatcacc acaactaact 
     5881 ttttcccgtt cctccatctc ttttatattt tttttctcga tcgagttcaa gagaaaaaaa 
     5941 aagaaaaagc aaaaagaaaa aaggaaagcg cgcctcgttc agaatgacac gtatagaatg 
     6001 atgcattacc ttgtcatctt cagtatcata ctgttcgtat acatacttac tgacattcat 
     6061 aggtatacat atatacacat gtatatatat cgtatgctgc agctttaaat aatcggtgtc 
     6121 actacataag aacacctttg gtggagggaa catcgttggt accattgggc gaggtggctt 
     6181 ctcttatggc aaccgcaaga gccttgaacg cactctcact acggtgatga tcattcttgc 
     6241 ctcgcagaca atcaacgtgg agggtaattc tgctagcctc tgcaaagctt tcaagaaaat 
     6301 gcgggatcat ctcgcaagag agatctccta ctttctccct ttgcaaacca agttcgacaa 
     6361 ctgcgtacgg cctgttcgaa agatctacca ccgctctgga aagtgcctca tccaaaggcg 
     6421 caaatcctga tccaaacctt tttactccac gcgccagtag ggcctcttta aaagcttgac 
     6481 cgagagcaat cccgcagtct tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc 
     6541 actcaacgat tagcgaccag ccggaatgct tggccagagc atgtatcata tggtccagaa 
     6601 accctatacc tgtgtggacg ttaatcactt gcgattgtgt ggcctgttct gctactgctt 
     6661 ctgcctcttt ttctgggaag atcgagtgct ctatcgctag gggaccaccc tttaaagaga 
     6721 tcgcaatctg aatcttggtt tcatttgtaa tacgctttac tagggctttc tgctctgtca 
     6781 tctttgcctt cgtttatctt gcctgctcat tttttagtat attcttcgaa gaaatcacat 
     6841 tactttatat aatgtataat tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag 
     6901 tcatccgcta ggtggaaaaa aaaaaatgaa aatcattacc gaggcataaa aaaatataga 
     6961 gtgtactaga ggaggccaag agtaatagaa aaagaaaatt gcgggaaagg actgtgttat 
     7021 gacttccctg actaatgccg tgttcaaacg atacctggca gtgactccta gcgctcacca 
     7081 agctcttaaa acgagaatta agaaaaagtc gtcatctttc gataagtttt tcccacagca 
     7141 aagcaatagt agaaaaacaa tgggaaacgt tgaatgaaga caaagcgtcg tggtttaaaa 
     7201 ggaaatacgc tcacgtacat gctagggaac aggaccgtgc agcggatcta atgaatccat 
     7261 ttgttagtta atagtttaaa tgtttttatc ggaagaggtt ttgtcatcac atcagcaatg 
     7321 ttcttcttgg tctcgatgta gtatacgtat aaattattac ctgatacttc atctctaagt 
     7381 ctcattgcct ttgtgccaaa aaatctgttt ctaaatttct cttcatttgt agacttaatt 
     7441 atactgatcg ttgatctact atcagtaagt aagcctttaa aaaaaaaaaa aaaaaaaaaa 
     7501 aaaaaaaaac ctgtaacaat agcaataccc caaataccta atgtagttcc agcaagcaag 
     7561 ctaaaaagta aagcaacaac ataactcacc cctgcatctg cagcttttgc ccgggcagcc 
     7621 tgctctgcct gtgttttctt taattgagca gtagaccatt tagcagttgc atgaatagct 
     7681 gcagcgtcac atcggataat aatgatggca gccattgtag aagtgccttt tgcatttcta 
     7741 gtctctttct cggtctagct agttttacta catcgcgaag atagaatctt agatcacact 
     7801 gcctttgctg agctggatca atagagtaac aaaagagtgg taaggcctcg ttaaaggaca 
     7861 aggacctgag cggaagtgta tcgtacagta gacggagtat actagtatag tctatagtcc 
     7921 gtggaattaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 
     7981 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 
     8041 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 
     8101 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 
     8161 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 
     8221 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 
     8281 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 
     8341 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc 
     8401 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 
     8461 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 
     8521 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 
     8581 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 
     8641 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt 
     8701 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 
     8761 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 
     8821 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 
     8881 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 
     8941 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 
     9001 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 
     9061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 
     9121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 
     9181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 
     9241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 
     9301 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 
     9361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 
     9421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 
     9481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 
     9541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 
     9601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 
     9661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 
     9721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 
     9781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 
     9841 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 
     9901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc 
     9961 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct 
    10021 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc 
    10081 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 
    10141 atccgcttac agacaagctg tgaccgtctc cggg 
//
