LOCUS pHZ5attR 11422 bp DNA circular 13-SEP-2005 SOURCE ORGANISM COMMENT Also known as pHZ5(NLS)attR2, pHZ5attR or pHZ5attR_2. Bank number 626. Finley et al, Gene. 2002 Feb 20;285(1-2):49-57. , PMID: 12039031 To combine ENTRY clone sequences with DESTINATION vectors to create EXPRESSION constructs use the sequence of an ENTRY clone that includes and is flanked by the 3' attB1 and 5' attB2 sequences, and the sequence of a DESTINATION vector that brackets, but does not include, the same two sequences: that includes the 5' and 3' common att sequences COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|208614226| COMMENT VNTDBDATE|385475403| COMMENT VNTNAME|pHZ5attR| COMMENT VNTAUTHORNAME|Finley Lab| COMMENT VNTAUTHORTEL|313-577-1627| COMMENT VNTAUTHORFAX|313-577-5218| COMMENT VNTAUTHORWWW|http://proteome.wayne.edu| COMMENT VNTAUTHORAD1|Center for Molecuar Medicine & Genetics| COMMENT VNTAUTHORAD2|Wayne State University School of Medicine| COMMENT VNTAUTHORAD3|540 East Canfield ave.| COMMENT VNTAUTHORAD4|Detroit, Michigan USA| FEATURES Location/Qualifiers CDS 906..1449 /vntifkey="4" /label=LexA\M1(TG)K2 /note="LexA coding region with two additional amino acids (TG) between M1 and K2." CDS 906..1508 /vntifkey="4" /label=LexA /note="LexA DBD (amino acids 1-202)" primer_bind 1470..1508 /vntifkey="28" /label=5'\RF126 /note="homologous to 5' end of RF126 primer" primer_bind 1491..1508 /vntifkey="28" /label=3'\3KF-BD /note="homologous to 3' end of 3KF-BD primer" primer 1141..1161 /vntifkey="27" /label=LexA\Central-s /note="LexA CDS primer, located in centre of DBD ORF" primer_bind 906..930 /vntifkey="28" /label=3'\RFx129 /note="priming site for the 3' end of RFx129. Amplifies all but ATG of LexA" primer 1482..1508 /vntifkey="27" /label=LexA\3' /note="sense primer for PCR amplification of LexA DBD plasmid inserts" primer 1484..1504 /vntifkey="27" /label=JZ45 /note="hybridize to the very end of LexA" CDS 1515..1538 /vntifkey="4" /label=NLS /note="Nuclear Localisation Sequence" primer 1243..1267 /vntifkey="27" /label=LexA-3PR /note="primer site for nested PCR of LexA-CS products and sequencing NRT junction" promoter 1..896 /vntifkey="29" /label=MAL61/62\promoter /note="MAL61/62 divergent promoter region, not including each ATG or PacI site upstream of MAL61 ATG" protein_bind complement(1605..1729) /vntifkey="31" /label=attR1 /note="lambda recombination site R1" CDS 1838..2497 /vntifkey="4" /label=CmR /note="Chloramphenicol resistance gene ORF" CDS 2617..2701 /vntifkey="4" /label=(ccdA) /note="inactivated ccdA bacterial gene" CDS 2839..3144 /vntifkey="4" /label=ccdB /note="ccdB antagonistic selection marker" protein_bind 3185..3309 /vntifkey="31" /label=attR2 /note="lambda recombination site R2" misc_recomb 1610..1624 /vntifkey="86" /label=5'\Common\att /note="central 15bp common to all 5' att sites" misc_recomb 3288..3306 /vntifkey="86" /label=3'\Common\att /note="central 15bp of attB2 site, common to all 3' att sites" misc_feature 1605..1609 /vntifkey="21" /label=5'\attB1 /note="5 most 5' bases of the attB1 site, are retained after LR reaction" primer_bind 1605..1626 /vntifkey="28" /label=R1\Priming\Site /note="binds primers for amplification of ccdB/CmR cassette for homologous repair" primer_bind complement(3288..3309) /vntifkey="28" /label=R2\Priming\Site /note="reverse primer site for PCR of ccdB/CmR cassette for homologous repair" primer_bind 3111..3131 /vntifkey="28" /label=attR2-S /note="sense primer for sequencing through attR2 site from within ccdB/CmR cassette" primer_bind 1873..1893 /vntifkey="28" /label=attR1-AS /note="anti-sense primer for sequencing attR1 site from within ccdB/CmR cassette" CDS complement(7176..7835) /vntifkey="4" /label=HIS3 /note="HIS3 gene, location according to Genbank file" CDS 9193..10053 /vntifkey="4" /label=ampR /note="ampicillin resistance gene" rep_origin <3952..>5848 /vntifkey="33" /label=2\µm\origin /note="2µm origin, the part that matches part of YEP24" primer_bind complement(3326..3350) /vntifkey="28" /label=JF16 /note="anti-sense ADH T primer" primer complement(3439..3456) /vntifkey="27" /label=CAS_02 /note="anti-sense ADH T primer, flanking JF16, works well with lexA Central-s" old_sequence <10065..>11277 /vntifkey="89" /label=pBr\ori /note="pBR backbone and ori" BASE COUNT 3277 a 2469 c 2564 g 3112 t ORIGIN 1 gtcttggatg taattcttat tgttatactg aatacgctaa aaccactcac aacaagtatg 61 gagtatattg tgtctcttta tatgctgagt acttatgcaa tatgcgctca ctcaggatga 121 aatgtacaca gccgaaagta tattgaaagc tgcctctgtg gaaacttcta tctaatcttg 181 tctccagatg tagactatga ggcctgaaga agtctttagg cacctgttgg agagtataag 241 gagactgcta caacaacgtc ttcccacaaa aaattatctg gaggccggta tgatacctgc 301 acaaaggtta agttacacat gaaaaagaaa ctgacataac tttgatctct gaaaatatct 361 tttcccctga gtagcttcac tgcttggata ccaataggaa tagaccttgg ctatagtaag 421 ttgcatctgt accgtagaga ttcttgcaac tcgcttaaac tctcgctttt agataatatt 481 tctccttatt gcgcgcttcg ttgaaaattt cgctaaacac ggggtttaag ttaaagttta 541 caggatttat ccggaaattt tcgcggaccc cacacaatta agaattggct cgaagagtga 601 taacgcatac ttttcttttc tttttttagt tcctagcgta cctaacgtag gtaacatgat 661 ttggatcgtg ggatgataca aacaacgtaa gatgactact tccttcctca attcttcttg 721 cagcatcatt ttcttgaggc gctctgggca aggtataaaa agttccatta atacgtctct 781 aaaaaattaa atcatccatc tcttaagcag tttttttgat aatctcaaat gtacatcagt 841 caagcgtaac taaattacat aaatgtacat cagtcaagcg taactaaatt acataaatga 901 ccggtaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt gatcacatca 961 gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc 1021 caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt 1081 ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg ccgctggtag 1141 gtcgtgtggc tgccggtgaa ccacttctgg cgcaacagca tattgaaggt cattatcagg 1201 tcgatccttc cttattcaag ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga 1261 tgaaagatat cggcattatg gatggtgact tgctggcagt gcataaaact caggatgtac 1321 gtaacggtca ggtcgttgtc gcacgtattg atgacgaagt taccgttaag cgcctgaaaa 1381 aacagggcaa taaagtcgaa ctgttgccag aaaatagcga gtttaaacca attgtcgtag 1441 atcttcgtca gcagagcttc accattgaag ggctggcggt tggggttatt cgcaacggcg 1501 actggctgga attggccccc aagaaaaaga gaaaggtgga attcccgggg atccgtcgac 1561 catggcggcc gctcgagtcg acctgcagcc aagctaattc cgggacaagt ttgtacaaaa 1621 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata 1681 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt 1741 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta 1801 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata 1861 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt 1921 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt 1981 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa 2041 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt 2101 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga 2161 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg 2221 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa 2281 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc 2341 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc 2401 gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt 2461 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa 2521 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga 2581 tatgtatacc cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca 2641 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg 2701 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa 2761 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg 2821 agaacaggga ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat 2881 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc 2941 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg 3001 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc 3061 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt 3121 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt 3181 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa 3241 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta 3301 caaagtggtg ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta 3361 aaacgaaaat tcttgttctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta 3421 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa atgcctgcaa 3481 atcgctcccc atttcaccca attgtagata tgctaactcc agcaatgagt tgatgaatct 3541 cggtgtgtat tttatgtcct cagaggacaa cacctgttgt aatcgttctt ccacacggat 3601 cgatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg 3661 aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc 3721 atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt 3781 cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta 3841 cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg 3901 gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc tagctttgaa 3961 gaaaaatgcg ccttattcaa tctttgctat aaaaaatggc ccaaaatctc acattggaag 4021 acatttgatg acctcatttc tttcaatgaa gggcctaacg gagttgacta atgttgtggg 4081 aaattggagc gataagcgtg cttctgccgt ggccaggaca acgtatactc atcagataac 4141 agcaatacct gatcactact tcgcactagt ttctcggtac tatgcatatg atccaatatc 4201 aaaggaaatg atagcattga aggatgagac taatccaatt gaggagtggc agcatataga 4261 acagctaaag ggtagtgctg aaggaagcat acgatacccc gcatggaatg ggataatatc 4321 acaggaggta ctagactacc tttcatccta cataaataga cgcatataag tacgcattta 4381 agcataaaca cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga 4441 tataggtgcg acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc 4501 gctcgttttc ggaaacgctt tgaagttcct attccgaagt tcctattctc tagaaagtat 4561 aggaacttca gagcgctttt gaaaaccaaa agcgctctga agacgcactt tcaaaaaacc 4621 aaaaacgcac cggactgtaa cgagctacta aaatattgcg aataccgctt ccacaaacat 4681 tgctcaaaag tatctctttg ctatatatct ctgtgctata tccctatata acctacccat 4741 ccacctttcg ctccttgaac ttgcatctaa actcgacctc tacatttttt atgtttatct 4801 ctagtattac tctttagaca aaaaaattgt agtaagaact attcatagag tgaatcgaaa 4861 acaatacgaa aatgtaaaca tttcctatac gtagtatata gagacaaaat agaagaaacc 4921 gttcataatt ttctgaccaa tgaagaatca tcaacgctat cactttctgt tcacaaagta 4981 tgcgcaatcc acatcggtat agaatataat cggggatgcc tttatcttga aaaaatgcac 5041 ccgcagcttc gctagtaatc agtaaacgcg ggaagtggag tcaggctttt tttatggaag 5101 agaaaataga caccaaagta gccttcttct aaccttaacg gacctacagt gcaaaaagtt 5161 atcaagagac tgcattatag agcgcacaaa ggagaaaaaa agtaatctaa gatgctttgt 5221 tagaaaaata gcgctctcgg gatgcatttt tgtagaacaa aaaagaagta tagattcttt 5281 gttggtaaaa tagcgctctc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct 5341 ttgtttgaaa aattagcgct ctcgcgttgc atttttgttt tacaaaaatg aagcacagat 5401 tcttcgttgg taaaatagcg ctttcgcgtt gcatttctgt tctgtaaaaa tgcagctcag 5461 attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttctacaa aatgaagcac 5521 agatgcttcg ttaacaaaga tatgctattg aagtgcaaga tggaaacgca gaaaatgaac 5581 cggggatgcg acgtgcaaga ttacctatgc aatagatgca atagtttctc caggaaccga 5641 aatacataca ttgtcttccg taaagcgcta gactatatat tattatacag gttcaaatat 5701 actatctgtt tcagggaaaa ctcccaggtt cggatgttca aaattcaatg atgggtaaca 5761 agtacgatcg taaatctgta aaacagtttg tcggatatta ggctgtatct cctcaaagcg 5821 tattcgaata tcattgagaa gctgcagcag gcgtgaagtt agacgacaac ttctctctgg 5881 aaacgcatac cgatattcag gctgctgcaa aggcacaggc tagtgcccgt gcgagtgcat 5941 ccggtaccac cccagatgct gtagtagctt ctggtagcac tgcaatgagc catgcttatc 6001 aagaaaacac aggttttggt actcgtccca tatatcttga catgcaagcc actacaccaa 6061 cagaccctag ggttttggat acgatgttga agttttatac gggactttat ggtaatcctc 6121 attccaacac tcactcttac ggttgggaaa caaatactgc tgtggaaaat gctagagctc 6181 acgtagcaaa gatgatcaat gccgacccca aggaaataat attcacttcg ggagcgaccg 6241 aatctaataa tatggttctt aagggtgtcc caagatttta taagaagact aagaaacaca 6301 tcatcaccac tagaacggaa cacaagtgtg tcttggaagc cgcacgggcc atgatgaagg 6361 agggatttga agtcactttc ctaaatgtgg acgatcaagg tcttatcgat ttgaaggaat 6421 tggaagatgc cattagacca gatacctgtc tcgtctctgt gatggctgtc aataatgaaa 6481 tcggtgtcat tcaacctatt aaagaaattg gagcaatttg tagaaagaat aagatcctcg 6541 gggacaccaa atatggcgat ctcggccttt tcgtttcttg gagctgggac atgtttgcca 6601 tcgatccatc taccaccaga acggccgtta gatctgctgc caccgttgtt tccaccgaag 6661 aaaccaccgt tgccgtaacc accacgacgg ttgttgctaa agaagctgcc accgccacgg 6721 ccaccgttgt agccgccgtt gttgttattg tagttgctac tgttatttct ggcacttctt 6781 ggttttcctc ttaagtgagg aggaacataa ccattctcgt tgttgtcgtt gatgcttaaa 6841 ttttgcactt gttcgctcag ttcagccata atatgaaatg cttttcttgt tgttcttacg 6901 gaataccact tgccacctat caccacaact aactttttcc cgttcctcca tctcttttat 6961 attttttttc tcgatcgagt tcaagagaaa aaaaaagaaa aagcaaaaag aaaaaaggaa 7021 agcgcgcctc gttcagaatg acacgtatag aatgatgcat taccttgtca tcttcagtat 7081 catactgttc gtatacatac ttactgacat tcataggtat acatatatac acatgtatat 7141 atatcgtatg ctgcagcttt aaataatcgg tgtcactaca taagaacacc tttggtggag 7201 ggaacatcgt tggtaccatt gggcgaggtg gcttctctta tggcaaccgc aagagccttg 7261 aacgcactct cactacggtg atgatcattc ttgcctcgca gacaatcaac gtggagggta 7321 attctgctag cctctgcaaa gctttcaaga aaatgcggga tcatctcgca agagagatct 7381 cctactttct ccctttgcaa accaagttcg acaactgcgt acggcctgtt cgaaagatct 7441 accaccgctc tggaaagtgc ctcatccaaa ggcgcaaatc ctgatccaaa cctttttact 7501 ccacgcgcca gtagggcctc tttaaaagct tgaccgagag caatcccgca gtcttcagtg 7561 gtgtgatggt cgtctatgtg taagtcacca atgcactcaa cgattagcga ccagccggaa 7621 tgcttggcca gagcatgtat catatggtcc agaaacccta tacctgtgtg gacgttaatc 7681 acttgcgatt gtgtggcctg ttctgctact gcttctgcct ctttttctgg gaagatcgag 7741 tgctctatcg ctaggggacc accctttaaa gagatcgcaa tctgaatctt ggtttcattt 7801 gtaatacgct ttactagggc tttctgctct gtcatctttg ccttcgttta tcttgcctgc 7861 tcatttttta gtatattctt cgaagaaatc acattacttt atataatgta taattcatta 7921 tgtgataatg ccaatcgcta agaaaaaaaa agagtcatcc gctaggtgga aaaaaaaaaa 7981 tgaaaatcat taccgaggca taaaaaaata tagagtgtac tagaggaggc caagagtaat 8041 agaaaaagaa aattgcggga aaggactgtg ttatgacttc cctgactaat gccgtgttca 8101 aacgatacct ggcagtgact cctagcgctc accaagctct taaaacgaga attaagaaaa 8161 agtcgtcatc tttcgataag tttttcccac agcaaagcaa tagtagaaaa acaatgggaa 8221 acgttgaatg aagacaaagc gtcgtggttt aaaaggaaat acgctcacgt acatgctagg 8281 gaacaggacc gtgcagcgga tctaatgaat ccatttgtta gttaatagtt taaatgtttt 8341 tatcggaaga ggttttgtca tcacatcagc aatgttcttc ttggtctcga tgtagtatac 8401 gtataaatta ttacctgata cttcatctct aagtctcatt gcctttgtgc caaaaaatct 8461 gtttctaaat ttctcttcat ttgtagactt aattatactg atcgttgatc tactatcagt 8521 aagtaagcct ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaacctgtaa caatagcaat 8581 accccaaata cctaatgtag ttccagcaag caagctaaaa agtaaagcaa caacataact 8641 cacccctgca tctgcagctt ttgcccgggc agcctgctct gcctgtgttt tctttaattg 8701 agcagtagac catttagcag ttgcatgaat agctgcagcg tcacatcgga taataatgat 8761 ggcagccatt gtagaagtgc cttttgcatt tctagtctct ttctcggtct agctagtttt 8821 actacatcgc gaagatagaa tcttagatca cactgccttt gctgagctgg atcaatagag 8881 taacaaaaga gtggtaaggc ctcgttaaag gacaaggacc tgagcggaag tgtatcgtac 8941 agtagacgga gtatactagt atagtctata gtccgtggaa ttaattcttg aagacgaaag 9001 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 9061 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 9121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 9181 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 9241 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 9301 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 9361 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 9421 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct 9481 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 9541 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 9601 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 9661 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 9721 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta 9781 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 9841 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 9901 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 9961 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 10021 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 10081 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 10141 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 10201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 10261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 10321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 10381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 10441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 10501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 10561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 10621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 10681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 10741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 10801 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 10861 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 10921 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 10981 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 11041 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat 11101 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg 11161 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 11221 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg 11281 atgatccggg atcgaagaaa tgatggtaaa tgaaatagga aatcaaggag catgaaggca 11341 aaagacaaat ataagggtcg aacgaaaaat aaagtgaaaa gtgttgatat gatgtatttg 11401 gctttgcggc gattaattaa ta //