LOCUS pJZ4 6664 bp DNA circular 4-SEP-2003 DEFINITION Yeast Two-hybrid AD fusion vector. SOURCE ORGANISM COMMENT Made by Julie Zhong, Finley lab A derivative of pJF1 (J. Fonfara and R. Finley, unpublished), which was derived from pJG4-5 ([Gyuris, 1993 #198] Gyuris et al., 1993, Cell 75, 791-803). Contains the CYC1 (instead of ADH1) transcription terminator. MCS includes two 8bp RE sites preceeding EcoRI and two following XhoI, for use in generating uni-directional deletions in conjunction with exonuclease III. One of the unique sites is Sse8387I. Zhong et al., 2003 COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|188651252| COMMENT VNTDBDATE|312843091| COMMENT VNTNAME|pJZ4| COMMENT VNTAUTHORNAME|Finley| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Yeast COMMENT VNTUDF|Finley Plasmid Bank Number|1|251| FEATURES Location/Qualifiers CDS 568..813 /codon_start=1 /transl_table=11 /product="B42 transcription activation domain-HA1 epitope tag fusion protein" /protein_id="AAB68652.1" /db_xref="PID:g2293122" /db_xref="GI:2293122" /vntifkey="4" /label=B42AD /note="B42 activation domain" CDS 814..843 /vntifkey="4" /label=HA\tag /note="encodes HA1 epitope tag" primer_bind 692..713 /vntifkey="28" /label=JZ23 /note="Primer JZ23, Ta ³ 65 C" primer_bind 785..806 /vntifkey="28" /label=BCO1 /note="Sense Primer for prey amplification, Ta ³ 65" primer_bind 557..576 /vntifkey="28" /label=CAS_01 /note="B42 AD sense Primer, flanks JZ23" primer_bind 814..831 /vntifkey="28" /label=3K-HA /note="homologous to 3' 18bp of 3K-HA primer" primer_bind 826..847 /vntifkey="28" /label=3'\RF125 /note="homologous to RF125, for vector conversion from pB42AD to pLexA, etc" CDS 532..567 /vntifkey="4" /label=NLS /note="SV40 NLS " primer_bind 808..828 /vntifkey="28" /label=JZ60 /note="foward sequencing/PCR primer annealing site" primer_bind complement(1156..1176) /vntifkey="28" /label=JZ5\3' /note="JZ5 anti-sense primer for CYC1 cloning" primer_bind 927..951 /vntifkey="28" /label=JZ4\3' /note="homologous to JZ4 sense primer for CYC1 terminator cloning" primer_bind complement(1013..1034) /vntifkey="28" /label=CAS_04 /note="anti-sense primer for CYC1 Terminator from pMyr (Stratagene)" primer_bind complement(947..969) /vntifkey="28" /label=JZ20 /note="anti-sense primer for CYC1 Terminator of pMyr" terminator 927..1176 /vntifkey="43" /label=CYC1\Terminator /note="CYC1 Terminator" primer_bind 1183..1212 /vntifkey="28" /label=JZ6\3' /note="Homologous to sense primer JZ6 for F1 Ori cloning" primer_bind complement(1534..1555) /vntifkey="28" /label=JZ7\3' /note="homologous to JZ7, anti-sense primer for F1 Ori cloning " rep_origin 1183..1555 /vntifkey="33" /label=F1\Ori /note="Origin of Replication" CDS complement(3578..4403) /vntifkey="4" /label=TRP1\Gene /note="Yeast TRP1 gene" gene 4613..5474 /gene="ampR" /vntifkey="60" CDS 4613..5473 /gene="ampR" /function="ampicillin resistance" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAB68653.1" /db_xref="PID:g2293123" /db_xref="GI:2293123" /vntifkey="4" /label=ampR rep_origin <1689..>3577 /vntifkey="33" /label=2\µm\Ori /note="Yeast 2µm origen of replication" rep_origin <5730..>6222 /vntifkey="33" /label=pUC\ori /note="pUC backbone; origin endpoints not correct" mat_peptide complement(3629..4303) /vntifkey="84" /label=TRP1\Peptide /note="TRP1 protein encoding sequence" primer_bind 35..56 /vntifkey="28" /label=CAS_03 /note="Sense primer for GAL1 Promoter" primer_bind 181..206 /vntifkey="28" /label=CAS_05 /note="ADH Promoter sense primer, est. Ta 68" promoter 1..521 /vntifkey="29" /label=GAL1\promoter /note="GAL1 promoter" BASE COUNT 1939 a 1485 c 1437 g 1803 t ORIGIN 1 ccattatctt agcctaaaaa aaccttctct ttggaacttt cagtaatacg cttaactgct 61 cattgctata ttgaagtacg gattagaagc cgccgagcgg gtgacagccc tccgaaggaa 121 gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc 181 gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg ttatgaagag 241 gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg aacgaatcaa attaacaacc 301 ataggatgat aatgcgatta gttttttagc cttatttctg gggtaattaa tcagcgaagc 361 gatgattttt gatctattaa cagatatata aatgcaaaaa ctgcataacc actttaacta 421 atactttcaa cattttcggt ttgtattact tcttattcaa atgtaataaa agtatcaaca 481 aaaaattgtt aatatacctc tatactttaa cgtcaaggag gaattaagct tatgggtgct 541 cctccaaaaa agaagagaaa ggtagctggt atcaataaag atatcgagga gtgcaatgcc 601 atcattgagc agtttatcga ctacctgcgc accggacagg agatgccgat ggaaatggcg 661 gatcaggcga ttaacgtggt gccgggcatg acgccgaaaa ccattcttca cgccgggccg 721 ccgatccagc ctgactggct gaaatcgaat ggttttcatg aaattgaagc ggatgttaac 781 gataccagcc tcttgctgag tggagatgcc tcctaccctt atgatgtgcc agattatgcc 841 tctcccgaat tgcctgcagg ctgtttaaac gaattcctag gcgcgccggc cctaggggcc 901 gagctcgagg gcgcgcctag gccggccatc atgtaattag ttatgtcacg cttacattca 961 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag 1021 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt 1081 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt 1141 gagaaggttt tgggacgctc gaaggcttta atttgcgtcg acaattgtaa acgttaatat 1201 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacg aatagcccga 1261 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 1321 agtttccaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaag 1381 ggtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 1441 gaggtgccgt aaagcagtaa atcggaaggg taaacggatg cccccattta gagcttgacg 1501 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cggggtcgac 1561 tagcggccgc ttcgacctgc agcaattctg aaccagtcct aaaacgagta aataggaccg 1621 gcaattcttc aagcaataaa caggaatacc aattattaaa agataactta gtcagatcgt 1681 acaataaagc tttgaagaaa aatgcgcctt attcaatctt tgctataaaa aatggcccaa 1741 aatctcacat tggaagacat ttgatgacct catttctttc aatgaagggc ctaacggagt 1801 tgactaatgt tgtgggaaat tggagcgata agcgtgcttc tgccgtggcc aggacaacgt 1861 atactcatca gataacagca atacctgatc actacttcgc actagtttct cggtactatg 1921 catatgatcc aatatcaaag gaaatgatag cattgaagga tgagactaat ccaattgagg 1981 agtggcagca tatagaacag ctaaagggta gtgctgaagg aagcatacga taccccgcat 2041 ggaatgggat aatatcacag gaggtactag actacctttc atcctacata aatagacgca 2101 tataagtacg catttaagca taaacacgca ctatgccgtt cttctcatgt atatatatat 2161 acaggcaaca cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc gcagctcgcg 2221 ttgcattttc ggaagcgctc gttttcggaa acgctttgaa gttcctattc cgaagttcct 2281 attctctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg ctctgaagac 2341 gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat attgcgaata 2401 ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt gctatatccc 2461 tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc gacctctaca 2521 ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta agaactattc 2581 atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag tatatagaga 2641 caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa cgctatcact 2701 ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg gatgccttta 2761 tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa gtggagtcag 2821 gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc ttaacggacc 2881 tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag aaaaaaagta 2941 atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta gaacaaaaaa 3001 gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa 3061 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca 3121 aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg 3181 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt 3241 ctacaaaatg aagcacagat gcttcgttaa caaagatatg ctattgaagt gcaagatgga 3301 aacgcagaaa atgaaccggg gatgcgacgt gcaagattac ctatgcaata gatgcaatag 3361 tttctccagg aaccgaaata catacattgt cttccgtaaa gcgctagact atatattatt 3421 atacaggttc aaatatacta tctgtttcag ggaaaactcc caggttcgga tgttcaaaat 3481 tcaatgatgg gtaacaagta cgatcgtaaa tctgtaaaac agtttgtcgg atattaggct 3541 gtatctcctc aaagcgtatt cgaatatcat tgagaagctg caggcaagtg cacaaacaat 3601 acttaaataa atactactca gtaataacct atttcttagc atttttgacg aaatttgcta 3661 ttttgttaga gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa 3721 cgccatttaa tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa 3781 aatgtaagct ttcggggctc tcttgccttc caacccagtc agaaatcgag ttccaatcca 3841 aaagttcacc tgtcccacct gcttctgaat caaacaaggg aataaacgaa tgaggtttct 3901 gtgaagctgc actgagtagt atgttgcagt cttttggaaa tacgagtctt ttaataactg 3961 gcaaaccgag gaactcttgg tattcttgcc acgactcatc tccatgcagt tggacgatat 4021 caatgccgta atcattgacc agagccaaaa catcctcctt aggttgatta cgaaacacgc 4081 caaccaagta tttcggagtg cctgaactat ttttatatgc ttttacaaga cttgaaattt 4141 tccttgcaat aaccgggtca attgttctct ttctattggg cacacatata atacccagca 4201 agtcagcatc ggaatctaga gcacattctg cggcctctgt gctctgcaag ccgcaaactt 4261 tcaccaatgg accagaacta cctgtgaaat taataacaga catactccaa gctgcctttg 4321 tgtgcttaat cacgtatact cacgtgctca atagtcacca atgccctccc tcttggccct 4381 ctccttttct tttttcgacc gaatttcttg aagacgaaag ggcctcgtga tacgcctatt 4441 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 4501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 4561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 4621 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 4681 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 4741 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 4801 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga 4861 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 4921 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 4981 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 5041 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 5101 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 5161 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 5221 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 5281 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 5341 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 5401 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 5461 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 5521 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 5581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 5641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 5701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 5761 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 5821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 5881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 5941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 6001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 6061 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 6121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 6181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 6241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 6301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 6361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc 6421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 6481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 6541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 6601 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattaat tcgagctcgg 6661 tacc //