LOCUS       pJZ4attR                8342 bp    DNA     circular UNA 02-APR-2009
DEFINITION  .
ACCESSION   urn.local.rfinley.1238695464997.1027
VERSION     urn.local.rfinley.1238695464997.1027
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             568..813
                     /Description="B42 activation domain"
                     /product="""B42 transcription activation domain-HA1
                     epitope tag fusion protein"
                     /db_xref="""GI:2293122"
                     /protein_id="""AAB68652.1"
                     /codon_start
                     /transl_table=1
                     /label="B42AD"
     CDS             814..843
                     /Description="encodes HA1 epitope tag"
                     /label="HA tag"
     primer_bind     692..713
                     /Description="Primer JZ23, Ta ³ 65 C"
                     /label="JZ23"
     primer_bind     785..806
                     /Description="Sense Primer for prey amplification, Ta ³
                     65"
                     /label="BCO1"
     primer_bind     557..576
                     /Description="B42 AD sense Primer, flanks JZ23"
                     /label="CAS_01"
     primer_bind     814..831
                     /Description="homologous to 3' 18bp of 3K-HA primer"
                     /label="3K-HA"
     primer_bind     826..847
                     /Description="homologous to RF125, for vector conversion
                     from pB42AD to pLexA, etc"
                     /label="3' RF125"
     CDS             532..567
                     /Description="SV40 NLS "
                     /label="NLS"
     primer_bind     808..828
                     /Description="foward sequencing/PCR primer annealing site"
                     /label="JZ60"
     primer_bind     35..56
                     /Description="Sense primer for GAL1 Promoter"
                     /label="CAS_03"
     primer_bind     181..206
                     /Description="ADH Promoter sense primer, est. Ta 68"
                     /label="CAS_05"
     misc_feature    1..521
                     /Description="GAL1 promoter"
                     /label="GAL1 promoter"
                     /note="Geneious type: promoter uukaryotic"
     attR1           complement(877..1001)
                     /Description="lambda recombination site R1"
                     /label="attR1"
     CDS             1110..1769
                     /Description="Chloramphenicol resistance gene ORF"
                     /label="CmR"
     CDS             1889..1973
                     /Description="inactivated ccdA bacterial gene"
                     /label="(ccdA)"
     CDS             2111..2416
                     /Description="ccdB antagonistic selection marker"
                     /label="ccdB"
     attR2           2457..2581
                     /Description="lambda recombination site R2"
                     /label="attR2"
     misc_recomb     882..896
                     /Description="central 15bp common to all 5' att sites"
                     /label="5' Common att"
     misc_recomb     2560..2578
                     /Description="central 15bp of attB2 site, common to all 3'
                     att sites"
                     /label="3' Common att"
     misc_feature    877..881
                     /Description="5 most 5' bases of the attB1 site, are
                     retained after LR reaction"
                     /label="5' attB1"
     primer_bind     877..898
                     /Description="binds primers for amplification of ccdB/CmR
                     cassette for homologous repair"
                     /label="R1 Priming Site"
     primer_bind     complement(2560..2581)
                     /Description="reverse primer site for PCR of ccdB/CmR
                     cassette for homologous repair"
                     /label="R2 Priming Site"
     attR2-S         2383..2403
                     /Description="sense primer for sequencing through attR2
                     site from within ccdB/CmR cassette"
                     /label="attR2-S"
     attR1-AS        1145..1165
                     /Description="anti-sense primer for sequencing attR1 site
                     from within ccdB/CmR cassette"
                     /label="attR1-AS"
     primer_bind     complement(2834..2854)
                     /Description="JZ5 anti-sense primer for CYC1 cloning"
                     /label="JZ5 3'"
     primer_bind     2605..2629
                     /Description="homologous to JZ4 sense primer for CYC1
                     terminator cloning"
                     /label="JZ4 3'"
     primer_bind     complement(2691..2712)
                     /Description="anti-sense primer for CYC1 Terminator from
                     pMyr (Stratagene)"
                     /label="CAS_04"
     primer_bind     complement(2625..2647)
                     /Description="anti-sense primer for CYC1 Terminator of
                     pMyr"
                     /label="JZ20"
     terminator      2605..2854
                     /Description="CYC1 Terminator"
                     /label="CYC1 Terminator"
     primer_bind     2861..2890
                     /Description="Homologous to sense primer JZ6 for F1 Ori
                     cloning"
                     /label="JZ6 3'"
     primer_bind     complement(3212..3233)
                     /Description="homologous to JZ7, anti-sense primer for F1
                     Ori cloning "
                     /label="JZ7 3'"
     rep_origin      2861..3233
                     /Description="Origin of Replication"
                     /label="F1 Ori"
     CDS             complement(5256..6081)
                     /Description="Yeast TRP1 gene"
                     /label="TRP1 Gene"
     gene            6291..7152
                     /gene="""ampR"
                     /label="Gene(ampR)_1"
     CDS             6291..7151
                     /product="""beta-lactamase"
                     /db_xref="""GI:2293123"
                     /gene="""ampR"
                     /protein_id="""AAB68653.1"
                     /codon_start
                     /function="""ampicillin resistance"
                     /transl_table=1
                     /label="ampR"
     rep_origin      3367..5255
                     /Description="Yeast 2µm origen of replication"
                     /label="2 µm Ori"
     rep_origin      7408..7900
                     /Description="pUC backbone; origin endpoints not correct"
                     /label="pUC ori"
     mat_peptide     complement(5307..5981)
                     /Description="TRP1 protein encoding sequence"
                     /label="TRP1 Peptide"
     primer_bind     777..796
                     /label="JH03"
     primer_bind     complement(2675..2695)
                     /label="JH04"
ORIGIN      
        1 ccattatctt agcctaaaaa aaccttctct ttggaacttt cagtaatacg cttaactgct
       61 cattgctata ttgaagtacg gattagaagc cgccgagcgg gtgacagccc tccgaaggaa
      121 gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc
      181 gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg ttatgaagag
      241 gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg aacgaatcaa attaacaacc
      301 ataggatgat aatgcgatta gttttttagc cttatttctg gggtaattaa tcagcgaagc
      361 gatgattttt gatctattaa cagatatata aatgcaaaaa ctgcataacc actttaacta
      421 atactttcaa cattttcggt ttgtattact tcttattcaa atgtaataaa agtatcaaca
      481 aaaaattgtt aatatacctc tatactttaa cgtcaaggag gaattaagct tatgggtgct
      541 cctccaaaaa agaagagaaa ggtagctggt atcaataaag atatcgagga gtgcaatgcc
      601 atcattgagc agtttatcga ctacctgcgc accggacagg agatgccgat ggaaatggcg
      661 gatcaggcga ttaacgtggt gccgggcatg acgccgaaaa ccattcttca cgccgggccg
      721 ccgatccagc ctgactggct gaaatcgaat ggttttcatg aaattgaagc ggatgttaac
      781 gataccagcc tcttgctgag tggagatgcc tcctaccctt atgatgtgcc agattatgcc
      841 tctcccgaat tgcctgcagg ctgtttaaac gaattcacaa gtttgtacaa aaaagctgaa
      901 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
      961 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
     1021 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
     1081 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     1141 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     1201 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     1261 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     1321 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     1381 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     1441 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     1501 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     1561 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1621 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     1681 ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     1741 actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat
     1801 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     1861 cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga cagttgacag
     1921 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     1981 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     2041 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     2101 gactggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     2161 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     2221 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     2281 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     2341 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     2401 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     2461 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     2521 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     2581 tctcgagggc gcgcctaggc cggccatcat gtaattagtt atgtcacgct tacattcacg
     2641 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     2701 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     2761 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     2821 gaaggttttg ggacgctcga aggctttaat ttgcgtcgac aattgtaaac gttaatattt
     2881 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaacgaa tagcccgaaa
     2941 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3001 tttccaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaggg
     3061 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     3121 ggtgccgtaa agcagtaaat cggaagggta aacggatgcc cccatttaga gcttgacggg
     3181 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg gggtcgacta
     3241 gcggccgctt cgacctgcag caattctgaa ccagtcctaa aacgagtaaa taggaccggc
     3301 aattcttcaa gcaataaaca ggaataccaa ttattaaaag ataacttagt cagatcgtac
     3361 aataaagctt tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa
     3421 tctcacattg gaagacattt gatgacctca tttctttcaa tgaagggcct aacggagttg
     3481 actaatgttg tgggaaattg gagcgataag cgtgcttctg ccgtggccag gacaacgtat
     3541 actcatcaga taacagcaat acctgatcac tacttcgcac tagtttctcg gtactatgca
     3601 tatgatccaa tatcaaagga aatgatagca ttgaaggatg agactaatcc aattgaggag
     3661 tggcagcata tagaacagct aaagggtagt gctgaaggaa gcatacgata ccccgcatgg
     3721 aatgggataa tatcacagga ggtactagac tacctttcat cctacataaa tagacgcata
     3781 taagtacgca tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac
     3841 aggcaacacg cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt
     3901 gcattttcgg aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat
     3961 tctctagaaa gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc
     4021 actttcaaaa aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc
     4081 gcttccacaa acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta
     4141 tataacctac ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt
     4201 ttttatgttt atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat
     4261 agagtgaatc gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca
     4321 aaatagaaga aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt
     4381 ctgttcacaa agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc
     4441 ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc
     4501 tttttttatg gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta
     4561 cagtgcaaaa agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat
     4621 ctaagatgct ttgttagaaa aatagcgctc tcgggatgca tttttgtaga acaaaaaaga
     4681 agtatagatt ctttgttggt aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat
     4741 gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttttacaaa
     4801 aatgaagcac agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta
     4861 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct
     4921 acaaaatgaa gcacagatgc ttcgttaaca aagatatgct attgaagtgc aagatggaaa
     4981 cgcagaaaat gaaccgggga tgcgacgtgc aagattacct atgcaataga tgcaatagtt
     5041 tctccaggaa ccgaaataca tacattgtct tccgtaaagc gctagactat atattattat
     5101 acaggttcaa atatactatc tgtttcaggg aaaactccca ggttcggatg ttcaaaattc
     5161 aatgatgggt aacaagtacg atcgtaaatc tgtaaaacag tttgtcggat attaggctgt
     5221 atctcctcaa agcgtattcg aatatcattg agaagctgca ggcaagtgca caaacaatac
     5281 ttaaataaat actactcagt aataacctat ttcttagcat ttttgacgaa atttgctatt
     5341 ttgttagagt cttttacacc atttgtctcc acacctccgc ttacatcaac accaataacg
     5401 ccatttaatc taagcgcatc accaacattt tctggcgtca gtccaccagc taacataaaa
     5461 tgtaagcttt cggggctctc ttgccttcca acccagtcag aaatcgagtt ccaatccaaa
     5521 agttcacctg tcccacctgc ttctgaatca aacaagggaa taaacgaatg aggtttctgt
     5581 gaagctgcac tgagtagtat gttgcagtct tttggaaata cgagtctttt aataactggc
     5641 aaaccgagga actcttggta ttcttgccac gactcatctc catgcagttg gacgatatca
     5701 atgccgtaat cattgaccag agccaaaaca tcctccttag gttgattacg aaacacgcca
     5761 accaagtatt tcggagtgcc tgaactattt ttatatgctt ttacaagact tgaaattttc
     5821 cttgcaataa ccgggtcaat tgttctcttt ctattgggca cacatataat acccagcaag
     5881 tcagcatcgg aatctagagc acattctgcg gcctctgtgc tctgcaagcc gcaaactttc
     5941 accaatggac cagaactacc tgtgaaatta ataacagaca tactccaagc tgcctttgtg
     6001 tgcttaatca cgtatactca cgtgctcaat agtcaccaat gccctccctc ttggccctct
     6061 ccttttcttt tttcgaccga atttcttgaa gacgaaaggg cctcgtgata cgcctatttt
     6121 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6181 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6361 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6421 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6481 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     6541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6601 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6661 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6721 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6841 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6901 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6961 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7021 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7081 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7141 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7201 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7441 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8041 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8101 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     8161 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     8221 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     8281 gataacaatt tcacacagga aacagctatg acatgattac gaattaattc gagctcggta
     8341 cc
//
