LOCUS pLexA(202+ 10144 bp DNA circular 26-FEB-2003 SOURCE ORGANISM COMMENT This vector was actually made by Doug Ruden before pEG202. pEG202 was made by Erica Golemis by inserting a polylinker into the mcs of pLexA(202+PL). For this file, the sequence of pEG202 was modified. COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|200318256| COMMENT VNTDBDATE|295365444| COMMENT VNTNAME|pLexA(202+PL)| COMMENT VNTAUTHORNAME|Finley| FEATURES Location/Qualifiers promoter 67..1511 /vntifkey="29" /label=ADH1\promoter /note="ADH1 promoter" CDS 1538..2143 /vntifkey="4" /label=LexA /note="LexA DBD (amino acids 1-202)" primer_bind 2105..2143 /vntifkey="28" /label=5'\RF126 /note="homologous to 5' end of RF126 primer" primer_bind 2126..2143 /vntifkey="28" /label=3'\3KF-BD /note="homologous to 3' end of 3KF-BD primer" primer 1776..1796 /vntifkey="27" /label=LexA\Central-s /note="LexA CDS primer, located in centre of DBD ORF" primer_bind 1541..1565 /vntifkey="28" /label=3'\RFx129 /note="priming site for the 3' end of RFx129. Amplifies all but ATG of LexA" primer 2117..2143 /vntifkey="27" /label=LexA\3' /note="sense primer for PCR amplification of LexA DBD plasmid inserts" primer 2119..2139 /vntifkey="27" /label=JZ45 /note="hybridize to the very end of LexA" primer_bind 1878..1902 /vntifkey="28" /label=LexA-3PR /note="primer for nested PCR in LexA-CS products, also for sequencing" CDS complement(6092..6751) /vntifkey="4" /label=HIS3 /note="HIS3 gene, location according to Genbank file" CDS 8109..8969 /vntifkey="4" /label=ampR /note="ampicillin resistance gene" rep_origin <2868..>4764 /vntifkey="33" /label=2\µm\origin /note="2µm origin, the part that matches part of YEP24" terminator 2187..2517 /vntifkey="43" /label=ADH\T /note="ADH Terminator region" primer_bind complement(2242..2266) /vntifkey="28" /label=JF16 /note="anti-sense ADH T primer" primer complement(2355..2372) /vntifkey="27" /label=CAS_02 /note="anti-sense ADH T primer, flanking JF16, works well with lexA Central-s" primer_bind complement(2183..2200) /vntifkey="28" /label=pEG202R /note="reverse primer for ADH terminator region" old_sequence <8981..>49 /vntifkey="89" /label=pBr\ori /note="pBR backbone and ori" BASE COUNT 2887 a 2236 c 2268 g 2753 t ORIGIN 1 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggatgatccg 61 ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg caaaagacaa 121 atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt tggctttgcg 181 gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg cggacccgcg 241 ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag ttttttgcgc 301 ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca ataagaatgc 361 cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa gttgccgaaa 421 gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac ttgcgagacg 481 cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga gacgcgcata 541 accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat aaatagacag 601 gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg 661 cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca catatattaa 721 ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt ttccgatttt 781 tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta caatatggac 841 ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct tcgttggtct 901 ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag acaagacata 961 atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac ataacgaact 1021 aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta ccctttttcc 1081 atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt tttttctttt 1141 ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac 1201 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg 1261 aggggtatct tcgaacacac gaaacttttt ccttccttca ttcacgcaca ctactctcta 1321 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaagtttgc 1381 cgctttgcta tcaagtataa atagacctgc aattattaat cttttgtttc ctcgtcattg 1441 ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct ataccaagca 1501 tacaatcaac tccaagcttg aattaattcc gggcggaatg aaagcgttaa cggccaggca 1561 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg 1621 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa 1681 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct 1741 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccact 1801 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttat tcaagccgaa 1861 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg 1921 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg 1981 tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt 2041 gccagaaaat agcgagttta aaccaattgt cgtagatctt cgtcagcaga gcttcaccat 2101 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctggaattcc cggggatccg 2161 tcgacctgca gccaagctaa ttccgggcga atttcttatg atttatgatt tttattatta 2221 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac 2281 gaaaattctt gttcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc 2341 atgaggtcgc tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg 2401 ctccccattt cacccaattg tagatatgct aactccagca atgagttgat gaatctcggt 2461 gtgtatttta tgtcctcaga ggacaacacc tgttgtaatc gttcttccac acggatcgat 2521 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc 2581 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag 2641 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga 2701 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc 2761 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc 2821 ctgactgcgt tagcaattta actgtgataa actaccgcat taaagctagc tttgaagaaa 2881 aatgcgcctt attcaatctt tgctataaaa aatggcccaa aatctcacat tggaagacat 2941 ttgatgacct catttctttc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat 3001 tggagcgata agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca 3061 atacctgatc actacttcgc actagtttct cggtactatg catatgatcc aatatcaaag 3121 gaaatgatag cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag 3181 ctaaagggta gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag 3241 gaggtactag actacctttc atcctacata aatagacgca tataagtacg catttaagca 3301 taaacacgca ctatgccgtt cttctcatgt atatatatat acaggcaaca cgcagatata 3361 ggtgcgacgt gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc 3421 gttttcggaa acgctttgaa gttcctattc cgaagttcct attctctaga aagtatagga 3481 acttcagagc gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa 3541 acgcaccgga ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct 3601 caaaagtatc tctttgctat atatctctgt gctatatccc tatataacct acccatccac 3661 ctttcgctcc ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag 3721 tattactctt tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa 3781 tacgaaaatg taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc 3841 ataattttct gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg 3901 caatccacat cggtatagaa tataatcggg gatgccttta tcttgaaaaa atgcacccgc 3961 agcttcgcta gtaatcagta aacgcgggaa gtggagtcag gcttttttta tggaagagaa 4021 aatagacacc aaagtagcct tcttctaacc ttaacggacc tacagtgcaa aaagttatca 4081 agagactgca ttatagagcg cacaaaggag aaaaaaagta atctaagatg ctttgttaga 4141 aaaatagcgc tctcgggatg catttttgta gaacaaaaaa gaagtataga ttctttgttg 4201 gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt 4261 ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca aaaatgaagc acagattctt 4321 cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg taaaaatgca gctcagattc 4381 tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ctacaaaatg aagcacagat 4441 gcttcgttaa caaagatatg ctattgaagt gcaagatgga aacgcagaaa atgaaccggg 4501 gatgcgacgt gcaagattac ctatgcaata gatgcaatag tttctccagg aaccgaaata 4561 catacattgt cttccgtaaa gcgctagact atatattatt atacaggttc aaatatacta 4621 tctgtttcag ggaaaactcc caggttcgga tgttcaaaat tcaatgatgg gtaacaagta 4681 cgatcgtaaa tctgtaaaac agtttgtcgg atattaggct gtatctcctc aaagcgtatt 4741 cgaatatcat tgagaagctg cagcaggcgt gaagttagac gacaacttct ctctggaaac 4801 gcataccgat attcaggctg ctgcaaaggc acaggctagt gcccgtgcga gtgcatccgg 4861 taccacccca gatgctgtag tagcttctgg tagcactgca atgagccatg cttatcaaga 4921 aaacacaggt tttggtactc gtcccatata tcttgacatg caagccacta caccaacaga 4981 ccctagggtt ttggatacga tgttgaagtt ttatacggga ctttatggta atcctcattc 5041 caacactcac tcttacggtt gggaaacaaa tactgctgtg gaaaatgcta gagctcacgt 5101 agcaaagatg atcaatgccg accccaagga aataatattc acttcgggag cgaccgaatc 5161 taataatatg gttcttaagg gtgtcccaag attttataag aagactaaga aacacatcat 5221 caccactaga acggaacaca agtgtgtctt ggaagccgca cgggccatga tgaaggaggg 5281 atttgaagtc actttcctaa atgtggacga tcaaggtctt atcgatttga aggaattgga 5341 agatgccatt agaccagata cctgtctcgt ctctgtgatg gctgtcaata atgaaatcgg 5401 tgtcattcaa cctattaaag aaattggagc aatttgtaga aagaataaga tcctcgggga 5461 caccaaatat ggcgatctcg gccttttcgt ttcttggagc tgggacatgt ttgccatcga 5521 tccatctacc accagaacgg ccgttagatc tgctgccacc gttgtttcca ccgaagaaac 5581 caccgttgcc gtaaccacca cgacggttgt tgctaaagaa gctgccaccg ccacggccac 5641 cgttgtagcc gccgttgttg ttattgtagt tgctactgtt atttctggca cttcttggtt 5701 ttcctcttaa gtgaggagga acataaccat tctcgttgtt gtcgttgatg cttaaatttt 5761 gcacttgttc gctcagttca gccataatat gaaatgcttt tcttgttgtt cttacggaat 5821 accacttgcc acctatcacc acaactaact ttttcccgtt cctccatctc ttttatattt 5881 tttttctcga tcgagttcaa gagaaaaaaa aagaaaaagc aaaaagaaaa aaggaaagcg 5941 cgcctcgttc agaatgacac gtatagaatg atgcattacc ttgtcatctt cagtatcata 6001 ctgttcgtat acatacttac tgacattcat aggtatacat atatacacat gtatatatat 6061 cgtatgctgc agctttaaat aatcggtgtc actacataag aacacctttg gtggagggaa 6121 catcgttggt accattgggc gaggtggctt ctcttatggc aaccgcaaga gccttgaacg 6181 cactctcact acggtgatga tcattcttgc ctcgcagaca atcaacgtgg agggtaattc 6241 tgctagcctc tgcaaagctt tcaagaaaat gcgggatcat ctcgcaagag agatctccta 6301 ctttctccct ttgcaaacca agttcgacaa ctgcgtacgg cctgttcgaa agatctacca 6361 ccgctctgga aagtgcctca tccaaaggcg caaatcctga tccaaacctt tttactccac 6421 gcgccagtag ggcctcttta aaagcttgac cgagagcaat cccgcagtct tcagtggtgt 6481 gatggtcgtc tatgtgtaag tcaccaatgc actcaacgat tagcgaccag ccggaatgct 6541 tggccagagc atgtatcata tggtccagaa accctatacc tgtgtggacg ttaatcactt 6601 gcgattgtgt ggcctgttct gctactgctt ctgcctcttt ttctgggaag atcgagtgct 6661 ctatcgctag gggaccaccc tttaaagaga tcgcaatctg aatcttggtt tcatttgtaa 6721 tacgctttac tagggctttc tgctctgtca tctttgcctt cgtttatctt gcctgctcat 6781 tttttagtat attcttcgaa gaaatcacat tactttatat aatgtataat tcattatgtg 6841 ataatgccaa tcgctaagaa aaaaaaagag tcatccgcta ggtggaaaaa aaaaaatgaa 6901 aatcattacc gaggcataaa aaaatataga gtgtactaga ggaggccaag agtaatagaa 6961 aaagaaaatt gcgggaaagg actgtgttat gacttccctg actaatgccg tgttcaaacg 7021 atacctggca gtgactccta gcgctcacca agctcttaaa acgagaatta agaaaaagtc 7081 gtcatctttc gataagtttt tcccacagca aagcaatagt agaaaaacaa tgggaaacgt 7141 tgaatgaaga caaagcgtcg tggtttaaaa ggaaatacgc tcacgtacat gctagggaac 7201 aggaccgtgc agcggatcta atgaatccat ttgttagtta atagtttaaa tgtttttatc 7261 ggaagaggtt ttgtcatcac atcagcaatg ttcttcttgg tctcgatgta gtatacgtat 7321 aaattattac ctgatacttc atctctaagt ctcattgcct ttgtgccaaa aaatctgttt 7381 ctaaatttct cttcatttgt agacttaatt atactgatcg ttgatctact atcagtaagt 7441 aagcctttaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaac ctgtaacaat agcaataccc 7501 caaataccta atgtagttcc agcaagcaag ctaaaaagta aagcaacaac ataactcacc 7561 cctgcatctg cagcttttgc ccgggcagcc tgctctgcct gtgttttctt taattgagca 7621 gtagaccatt tagcagttgc atgaatagct gcagcgtcac atcggataat aatgatggca 7681 gccattgtag aagtgccttt tgcatttcta gtctctttct cggtctagct agttttacta 7741 catcgcgaag atagaatctt agatcacact gcctttgctg agctggatca atagagtaac 7801 aaaagagtgg taaggcctcg ttaaaggaca aggacctgag cggaagtgta tcgtacagta 7861 gacggagtat actagtatag tctatagtcc gtggaattaa ttcttgaaga cgaaagggcc 7921 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 7981 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 8041 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 8101 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 8161 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 8221 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 8281 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 8341 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 8401 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 8461 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 8521 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 8581 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 8641 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 8701 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 8761 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 8821 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 8881 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 8941 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 9001 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 9061 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 9121 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 9181 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 9241 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 9301 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 9361 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 9421 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 9481 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 9541 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 9601 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 9661 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 9721 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 9781 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 9841 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 9901 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 9961 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac 10021 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga 10081 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 10141 cggg //