LOCUS pNLexAattR 11863 bp DNA circular 4-SEP-2003 SOURCE ORGANISM COMMENT Alternative name: pNLex(NLS)attR_2 Finley et al., Gene 285, 49-57 To combine ENTRY clone sequences with DESTINATION vectors to create EXPRESSION constructsuse the sequence of an ENTRY clone that includes and is flanked by the 3' attB1 and 5' attB2 sequences, and the sequence of a DESTINATION vector that brackets, but does not include, the same two sequences: that includes the 5' and 3' common att sequences COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|208614249| COMMENT VNTDBDATE|312834197| COMMENT VNTNAME|pNLexAattR| COMMENT VNTAUTHORNAME|Finley| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Yeast FEATURES Location/Qualifiers promoter 67..1511 /vntifkey="29" /label=ADH1\promoter /note="ADH1 promoter" CDS 1538..2143 /vntifkey="4" /label=LexA /note="LexA DBD (amino acids 1-202)" primer_bind 2105..2143 /vntifkey="28" /label=5'\RF126 /note="homologous to 5' end of RF126 primer" primer_bind 2126..2143 /vntifkey="28" /label=3'\3KF-BD /note="homologous to 3' end of 3KF-BD primer" primer 1776..1796 /vntifkey="27" /label=LexA\Central-s /note="LexA CDS primer, located in centre of DBD ORF" primer_bind 1541..1565 /vntifkey="28" /label=3'\RFx129 /note="priming site for the 3' end of RFx129. Amplifies all but ATG of LexA" primer 2117..2143 /vntifkey="27" /label=LexA\3' /note="sense primer for PCR amplification of LexA DBD plasmid inserts" primer 2119..2139 /vntifkey="27" /label=JZ45 /note="hybridize to the very end of LexA" primer_bind 1878..1902 /vntifkey="28" /label=LexA-3PR /note="primer for nested PCR in LexA-CS products, also for sequencing" CDS 2150..2173 /vntifkey="4" /label=NLS /note="Nuclear Localisation Sequence" protein_bind complement(2174..2298) /vntifkey="31" /label=attR1 /note="lambda recombination site R1" CDS 2407..3066 /vntifkey="4" /label=CmR /note="Chloramphenicol resistance gene ORF" CDS 3186..3270 /vntifkey="4" /label=(ccdA) /note="inactivated ccdA bacterial gene" CDS 3408..3713 /vntifkey="4" /label=ccdB /note="ccdB antagonistic selection marker" protein_bind 3754..3878 /vntifkey="31" /label=attR2 /note="lambda recombination site R2" misc_recomb 2179..2193 /vntifkey="86" /label=5'\Common\att /note="central 15bp common to all 5' att sites" misc_recomb 3857..3875 /vntifkey="86" /label=3'\Common\att /note="central 15bp of attB2 site, common to all 3' att sites" misc_feature 2174..2178 /vntifkey="21" /label=5'\attB1 /note="5 most 5' bases of the attB1 site, are retained after LR reaction" primer_bind 2174..2195 /vntifkey="28" /label=R1\Priming\Site /note="binds primers for amplification of ccdB/CmR cassette for homologous repair" primer_bind complement(3857..3878) /vntifkey="28" /label=R2\Priming\Site /note="reverse primer site for PCR of ccdB/CmR cassette for homologous repair" primer_bind 3680..3700 /vntifkey="28" /label=attR2-S /note="sense primer for sequencing through attR2 site from within ccdB/CmR cassette" primer_bind 2442..2462 /vntifkey="28" /label=attR1-AS /note="anti-sense primer for sequencing attR1 site from within ccdB/CmR cassette" CDS complement(7811..8470) /vntifkey="4" /label=HIS3 /note="HIS3 gene, location according to Genbank file" CDS 9828..10688 /vntifkey="4" /label=ampR /note="ampicillin resistance gene" rep_origin <4587..>6483 /vntifkey="33" /label=2\µm\origin /note="2µm origin, the part that matches part of YEP24" terminator 3906..4236 /vntifkey="43" /label=ADH\T /note="ADH Terminator region" primer_bind complement(3961..3985) /vntifkey="28" /label=JF16 /note="anti-sense ADH T primer" primer complement(4074..4091) /vntifkey="27" /label=CAS_02 /note="anti-sense ADH T primer, flanking JF16, works well with lexA Central-s" primer_bind complement(3902..3919) /vntifkey="28" /label=pEG202R /note="reverse primer for ADH terminator region" old_sequence <10700..>49 /vntifkey="89" /label=pBr\ori /note="pBR backbone and ori" BASE COUNT 3378 a 2583 c 2673 g 3229 t ORIGIN 1 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggatgatccg 61 ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg caaaagacaa 121 atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt tggctttgcg 181 gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg cggacccgcg 241 ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag ttttttgcgc 301 ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca ataagaatgc 361 cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa gttgccgaaa 421 gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac ttgcgagacg 481 cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga gacgcgcata 541 accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat aaatagacag 601 gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg 661 cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca catatattaa 721 ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt ttccgatttt 781 tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta caatatggac 841 ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct tcgttggtct 901 ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag acaagacata 961 atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac ataacgaact 1021 aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta ccctttttcc 1081 atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt tttttctttt 1141 ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac 1201 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg 1261 aggggtatct tcgaacacac gaaacttttt ccttccttca ttcacgcaca ctactctcta 1321 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaagtttgc 1381 cgctttgcta tcaagtataa atagacctgc aattattaat cttttgtttc ctcgtcattg 1441 ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct ataccaagca 1501 tacaatcaac tccaagcttg aattaattcc gggcggaatg aaagcgttaa cggccaggca 1561 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg 1621 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa 1681 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct 1741 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccact 1801 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttat tcaagccgaa 1861 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg 1921 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg 1981 tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt 2041 gccagaaaat agcgagttta aaccaattgt cgtagatctt cgtcagcaga gcttcaccat 2101 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctggaattgg cccccaagaa 2161 aaagagaaag gtgacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa 2221 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca 2281 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc 2341 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa 2401 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt 2461 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag 2521 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc 2581 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa 2641 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa 2701 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac 2761 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt 2821 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta 2881 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg 2941 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc 3001 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg 3061 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct 3121 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt 3181 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg 3241 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg 3301 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt 3361 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg 3421 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta 3481 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata 3541 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga 3601 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc 3661 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag 3721 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta 3781 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca 3841 ttttacgttt ctcgttcagc tttcttgtac aaagtggtgt cgacctgcag ccaagctaat 3901 tccgggcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa 3961 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattcttg ttcttgagta 4021 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc 4081 acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc acccaattgt 4141 agatatgcta actccagcaa tgagttgatg aatctcggtg tgtattttat gtcctcagag 4201 gacaacacct gttgtaatcg ttcttccaca cggatcgatc cacaggacgg gtgtggtcgc 4261 catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc 4321 aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag 4381 cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag 4441 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag 4501 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa 4561 ctgtgataaa ctaccgcatt aaagctagct ttgaagaaaa atgcgcctta ttcaatcttt 4621 gctataaaaa atggcccaaa atctcacatt ggaagacatt tgatgacctc atttctttca 4681 atgaagggcc taacggagtt gactaatgtt gtgggaaatt ggagcgataa gcgtgcttct 4741 gccgtggcca ggacaacgta tactcatcag ataacagcaa tacctgatca ctacttcgca 4801 ctagtttctc ggtactatgc atatgatcca atatcaaagg aaatgatagc attgaaggat 4861 gagactaatc caattgagga gtggcagcat atagaacagc taaagggtag tgctgaagga 4921 agcatacgat accccgcatg gaatgggata atatcacagg aggtactaga ctacctttca 4981 tcctacataa atagacgcat ataagtacgc atttaagcat aaacacgcac tatgccgttc 5041 ttctcatgta tatatatata caggcaacac gcagatatag gtgcgacgtg aacagtgagc 5101 tgtatgtgcg cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag 5161 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaaaa 5221 ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc 5281 tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata 5341 tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca 5401 tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt agacaaaaaa 5461 attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc 5521 tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg accaatgaag 5581 aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc ggtatagaat 5641 ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa 5701 acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca aagtagcctt 5761 cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat tatagagcgc 5821 acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct ctcgggatgc 5881 atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg ctctcgcgtt 5941 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc 6001 gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa tagcgctttc 6061 gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct 6121 ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttaac aaagatatgc 6181 tattgaagtg caagatggaa acgcagaaaa tgaaccgggg atgcgacgtg caagattacc 6241 tatgcaatag atgcaatagt ttctccagga accgaaatac atacattgtc ttccgtaaag 6301 cgctagacta tatattatta tacaggttca aatatactat ctgtttcagg gaaaactccc 6361 aggttcggat gttcaaaatt caatgatggg taacaagtac gatcgtaaat ctgtaaaaca 6421 gtttgtcgga tattaggctg tatctcctca aagcgtattc gaatatcatt gagaagctgc 6481 agcaggcgtg aagttagacg acaacttctc tctggaaacg cataccgata ttcaggctgc 6541 tgcaaaggca caggctagtg cccgtgcgag tgcatccggt accaccccag atgctgtagt 6601 agcttctggt agcactgcaa tgagccatgc ttatcaagaa aacacaggtt ttggtactcg 6661 tcccatatat cttgacatgc aagccactac accaacagac cctagggttt tggatacgat 6721 gttgaagttt tatacgggac tttatggtaa tcctcattcc aacactcact cttacggttg 6781 ggaaacaaat actgctgtgg aaaatgctag agctcacgta gcaaagatga tcaatgccga 6841 ccccaaggaa ataatattca cttcgggagc gaccgaatct aataatatgg ttcttaaggg 6901 tgtcccaaga ttttataaga agactaagaa acacatcatc accactagaa cggaacacaa 6961 gtgtgtcttg gaagccgcac gggccatgat gaaggaggga tttgaagtca ctttcctaaa 7021 tgtggacgat caaggtctta tcgatttgaa ggaattggaa gatgccatta gaccagatac 7081 ctgtctcgtc tctgtgatgg ctgtcaataa tgaaatcggt gtcattcaac ctattaaaga 7141 aattggagca atttgtagaa agaataagat cctcggggac accaaatatg gcgatctcgg 7201 ccttttcgtt tcttggagct gggacatgtt tgccatcgat ccatctacca ccagaacggc 7261 cgttagatct gctgccaccg ttgtttccac cgaagaaacc accgttgccg taaccaccac 7321 gacggttgtt gctaaagaag ctgccaccgc cacggccacc gttgtagccg ccgttgttgt 7381 tattgtagtt gctactgtta tttctggcac ttcttggttt tcctcttaag tgaggaggaa 7441 cataaccatt ctcgttgttg tcgttgatgc ttaaattttg cacttgttcg ctcagttcag 7501 ccataatatg aaatgctttt cttgttgttc ttacggaata ccacttgcca cctatcacca 7561 caactaactt tttcccgttc ctccatctct tttatatttt ttttctcgat cgagttcaag 7621 agaaaaaaaa agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca gaatgacacg 7681 tatagaatga tgcattacct tgtcatcttc agtatcatac tgttcgtata catacttact 7741 gacattcata ggtatacata tatacacatg tatatatatc gtatgctgca gctttaaata 7801 atcggtgtca ctacataaga acacctttgg tggagggaac atcgttggta ccattgggcg 7861 aggtggcttc tcttatggca accgcaagag ccttgaacgc actctcacta cggtgatgat 7921 cattcttgcc tcgcagacaa tcaacgtgga gggtaattct gctagcctct gcaaagcttt 7981 caagaaaatg cgggatcatc tcgcaagaga gatctcctac tttctccctt tgcaaaccaa 8041 gttcgacaac tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa agtgcctcat 8101 ccaaaggcgc aaatcctgat ccaaaccttt ttactccacg cgccagtagg gcctctttaa 8161 aagcttgacc gagagcaatc ccgcagtctt cagtggtgtg atggtcgtct atgtgtaagt 8221 caccaatgca ctcaacgatt agcgaccagc cggaatgctt ggccagagca tgtatcatat 8281 ggtccagaaa ccctatacct gtgtggacgt taatcacttg cgattgtgtg gcctgttctg 8341 ctactgcttc tgcctctttt tctgggaaga tcgagtgctc tatcgctagg ggaccaccct 8401 ttaaagagat cgcaatctga atcttggttt catttgtaat acgctttact agggctttct 8461 gctctgtcat ctttgccttc gtttatcttg cctgctcatt ttttagtata ttcttcgaag 8521 aaatcacatt actttatata atgtataatt cattatgtga taatgccaat cgctaagaaa 8581 aaaaaagagt catccgctag gtggaaaaaa aaaaatgaaa atcattaccg aggcataaaa 8641 aaatatagag tgtactagag gaggccaaga gtaatagaaa aagaaaattg cgggaaagga 8701 ctgtgttatg acttccctga ctaatgccgt gttcaaacga tacctggcag tgactcctag 8761 cgctcaccaa gctcttaaaa cgagaattaa gaaaaagtcg tcatctttcg ataagttttt 8821 cccacagcaa agcaatagta gaaaaacaat gggaaacgtt gaatgaagac aaagcgtcgt 8881 ggtttaaaag gaaatacgct cacgtacatg ctagggaaca ggaccgtgca gcggatctaa 8941 tgaatccatt tgttagttaa tagtttaaat gtttttatcg gaagaggttt tgtcatcaca 9001 tcagcaatgt tcttcttggt ctcgatgtag tatacgtata aattattacc tgatacttca 9061 tctctaagtc tcattgcctt tgtgccaaaa aatctgtttc taaatttctc ttcatttgta 9121 gacttaatta tactgatcgt tgatctacta tcagtaagta agcctttaaa aaaaaaaaaa 9181 aaaaaaaaaa aaaaaaaacc tgtaacaata gcaatacccc aaatacctaa tgtagttcca 9241 gcaagcaagc taaaaagtaa agcaacaaca taactcaccc ctgcatctgc agcttttgcc 9301 cgggcagcct gctctgcctg tgttttcttt aattgagcag tagaccattt agcagttgca 9361 tgaatagctg cagcgtcaca tcggataata atgatggcag ccattgtaga agtgcctttt 9421 gcatttctag tctctttctc ggtctagcta gttttactac atcgcgaaga tagaatctta 9481 gatcacactg cctttgctga gctggatcaa tagagtaaca aaagagtggt aaggcctcgt 9541 taaaggacaa ggacctgagc ggaagtgtat cgtacagtag acggagtata ctagtatagt 9601 ctatagtccg tggaattaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat 9661 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 9721 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 9781 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 9841 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 9901 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 9961 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 10021 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 10081 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 10141 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 10201 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 10261 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 10321 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg 10381 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 10441 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 10501 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 10561 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 10621 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 10681 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 10741 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 10801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 10861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 10921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 10981 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 11041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 11101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 11161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 11221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 11281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 11341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 11401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 11461 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 11521 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 11581 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 11641 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 11701 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 11761 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 11821 gctcccggca tccgcttaca gacaagctgt gaccgtctcc ggg //