LOCUS pRF4-6o 6198 bp DNA circular 23-FEB-2004
SOURCE
ORGANISM
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to 527 (right terminus) of pRF4-5o (which excludes the B42 AD). Added
CTATAATG. Sequence at the Ga1p-ATG-Epitope tag junction matches
the sequenced part of pRF4-6NL except for an A at 516 which did
not show up in the sequenced sequence. The
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|331061284|
COMMENT VNTDBDATE|331061337|
COMMENT VNTNAME|pRF4-6o|
COMMENT VNTAUTHORNAME|Finley|
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FEATURES Location/Qualifiers
promoter 1..521
/vntifkey="29"
/label=GAL1\promoter
/note="GAL1 promoter"
CDS 542..571
/vntifkey="4"
/label=HA\tag
/note="encodes HA1 epitope tag"
CDS complement(3112..3937)
/vntifkey="4"
/label=TRP1\Gene
/note="Yeast TRP1 gene"
gene 4147..5008
/gene="ampR"
/vntifkey="60"
CDS 4147..5007
/gene="ampR"
/function="ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAB68653.1"
/db_xref="PID:g2293123"
/db_xref="GI:2293123"
/vntifkey="4"
/label=ampR
rep_origin <1223..>3111
/vntifkey="33"
/label=2\µm\Ori
/note="Yeast 2µm origen of replication"
rep_origin <5264..>5756
/vntifkey="33"
/label=pUC\ori
/note="pUC backbone; origin endpoints not correct"
terminator 636..1073
/vntifkey="43"
/label=ADH\Terminator
/note="Transcrption terminator from yeast ADH1"
primer_bind complement(699..723)
/vntifkey="28"
/label=3'\JZ24
/note="Anti-sense primer for Prey amplification, Ta ³65 C"
primer_bind complement(654..674)
/vntifkey="28"
/label=BCO2
/note="Anti-sense primer for Prey amplification, Ta³ 65 C"
primer_bind complement(919..936)
/vntifkey="28"
/label=CAS_02
/note="Anti-sense primer for ADH Terminator, flanks JZ24"
primer_bind 542..559
/vntifkey="28"
/label=3K-HA
/note="homologous to 3' 18bp of 3K-HA primer"
mat_peptide complement(3163..3837)
/vntifkey="84"
/label=TRP1\Peptide
/note="TRP1 protein encoding sequence"
primer_bind 554..575
/vntifkey="28"
/label=3'\RF125
/note="homologous to RF125, for vector conversion from pB42AD to pLexA, etc"
primer_bind 35..56
/vntifkey="28"
/label=CAS_03
/note="Sense primer for GAL1 Promoter"
primer_bind 181..206
/vntifkey="28"
/label=CAS_05
/note="ADH Promoter sense primer, est. Ta 68"
primer_bind complement(806..830)
/vntifkey="28"
/label=JF16
/note="anti-sense primer for vector conversion"
primer_bind complement(916..939)
/vntifkey="28"
/label=CAS_06
/note="anti-sense primer for ADH Terminator region. Higher Ta than CAS_02"
primer_bind 536..556
/vntifkey="28"
/label=JZ60
/note="foward sequencing/PCR primer annealing site"
BASE COUNT 1801 a 1385 c 1294 g 1718 t
ORIGIN
1 ccattatctt agcctaaaaa aaccttctct ttggaacttt cagtaatacg cttaactgct
61 cattgctata ttgaagtacg gattagaagc cgccgagcgg gtgacagccc tccgaaggaa
121 gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc
181 gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg ttatgaagag
241 gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg aacgaatcaa attaacaacc
301 ataggatgat aatgcgatta gttttttagc cttatttctg gggtaattaa tcagcgaagc
361 gatgattttt gatctattaa cagatatata aatgcaaaaa ctgcataacc actttaacta
421 atactttcaa cattttcggt ttgtattact tcttattcaa atgtaataaa agtatcaaca
481 aaaaattgtt aatatacctc tatactttaa cgtcaaggag gaattaacta taatggcctc
541 ctacccttat gatgtgccag attatgcctc tcccgaattc ctaggcgcgc cggccctagg
601 ggccgagctc gagaagcttt ggacttcttc gccagaggtt tggtcaagtc tccaatcaag
661 gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt
721 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
781 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
841 aaacgaaaat tcttgttctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
901 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa atgcctgcaa
961 atcgctcccc atttcaccca attgtagata tgctaactcc agcaatgagt tgatgaatct
1021 cggtgtgtat tttatgtcct cagaggacaa cacctgttgt aatcgttctt ccacacggat
1081 cctctagagt cgactagcgg ccgcttcgac ctgcagcaat tctgaaccag tcctaaaacg
1141 agtaaatagg accggcaatt cttcaagcaa taaacaggaa taccaattat taaaagataa
1201 cttagtcaga tcgtacaata aagctttgaa gaaaaatgcg ccttattcaa tctttgctat
1261 aaaaaatggc ccaaaatctc acattggaag acatttgatg acctcatttc tttcaatgaa
1321 gggcctaacg gagttgacta atgttgtggg aaattggagc gataagcgtg cttctgccgt
1381 ggccaggaca acgtatactc atcagataac agcaatacct gatcactact tcgcactagt
1441 ttctcggtac tatgcatatg atccaatatc aaaggaaatg atagcattga aggatgagac
1501 taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg aaggaagcat
1561 acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc tttcatccta
1621 cataaataga cgcatataag tacgcattta agcataaaca cgcactatgc cgttcttctc
1681 atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat
1741 gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct
1801 attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt gaaaaccaaa
1861 agcgctctga agacgcactt tcaaaaaacc aaaaacgcac cggactgtaa cgagctacta
1921 aaatattgcg aataccgctt ccacaaacat tgctcaaaag tatctctttg ctatatatct
1981 ctgtgctata tccctatata acctacccat ccacctttcg ctccttgaac ttgcatctaa
2041 actcgacctc tacatttttt atgtttatct ctagtattac tctttagaca aaaaaattgt
2101 agtaagaact attcatagag tgaatcgaaa acaatacgaa aatgtaaaca tttcctatac
2161 gtagtatata gagacaaaat agaagaaacc gttcataatt ttctgaccaa tgaagaatca
2221 tcaacgctat cactttctgt tcacaaagta tgcgcaatcc acatcggtat agaatataat
2281 cggggatgcc tttatcttga aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg
2341 ggaagtggag tcaggctttt tttatggaag agaaaataga caccaaagta gccttcttct
2401 aaccttaacg gacctacagt gcaaaaagtt atcaagagac tgcattatag agcgcacaaa
2461 ggagaaaaaa agtaatctaa gatgctttgt tagaaaaata gcgctctcgg gatgcatttt
2521 tgtagaacaa aaaagaagta tagattcttt gttggtaaaa tagcgctctc gcgttgcatt
2581 tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc
2641 atttttgttt tacaaaaatg aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt
2701 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
2761 gttgcatttt tgttctacaa aatgaagcac agatgcttcg ttaacaaaga tatgctattg
2821 aagtgcaaga tggaaacgca gaaaatgaac cggggatgcg acgtgcaaga ttacctatgc
2881 aatagatgca atagtttctc caggaaccga aatacataca ttgtcttccg taaagcgcta
2941 gactatatat tattatacag gttcaaatat actatctgtt tcagggaaaa ctcccaggtt
3001 cggatgttca aaattcaatg atgggtaaca agtacgatcg taaatctgta aaacagtttg
3061 tcggatatta ggctgtatct cctcaaagcg tattcgaata tcattgagaa gctgcaggca
3121 agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct tagcattttt
3181 gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac ctccgcttac
3241 atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg gcgtcagtcc
3301 accagctaac ataaaatgta agctttcggg gctctcttgc cttccaaccc agtcagaaat
3361 cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa
3421 cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag
3481 tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg
3541 cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg
3601 attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac
3661 aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca
3721 tataataccc agcaagtcag catcggaatc tagagcacat tctgcggcct ctgtgctctg
3781 caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa cagacatact
3841 ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc accaatgccc
3901 tccctcttgg ccctctcctt ttcttttttc gaccgaattt cttgaagacg aaagggcctc
3961 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
4021 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
4081 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
4141 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
4201 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
4261 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
4321 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
4381 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
4441 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
4501 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
4561 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
4621 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
4681 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
4741 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
4801 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
4861 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
4921 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
4981 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
5041 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
5101 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
5161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
5221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
5281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
5341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
5401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
5461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
5521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
5581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
5641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
5701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
5761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
5821 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
5881 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
5941 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
6001 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
6061 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
6121 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
6181 taattcgagc tcggtacc
//