LOCUS       pTLJ03       6101 bp    DNA   circular            14-SEP-2004
DEFINITION  Complementary copy of pTLJ03.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|293458310|
COMMENT     VNTDBDATE|349639463|
COMMENT     VNTNAME|pTLJ03|
COMMENT     VNTAUTHORNAME|Finley|
FEATURES             Location/Qualifiers
     CDS             1101..1958
                     /vntifkey="4"
                     /label=Ap
     CDS             complement(4656..5735)
                     /vntifkey="4"
                     /label=lacI
                     /note="encodes for lacI"
     rep_origin      complement(2719..2719)
                     /vntifkey="33"
                     /label=ori
                     /note="pBR322 replication origin"
     misc_feature    38..38
                     /vntifkey="21"
                     /label=T7\transcription\start
                     /note="T7 transcription start"
     promoter        21..37
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="T7 promoter"
     RBS             complement(94..101)
                     /vntifkey="32"
                     /label=RBS
     misc_recomb     874..894
                     /vntifkey="86"
                     /label=3RT1
                     /note="3RT1 recombination tag"
     misc_recomb     841..861
                     /vntifkey="86"
                     /label=5RT1
                     /note="5RT1 recombination tag"
     loci            823..840
                     /vntifkey="18"
                     /label=6His
                     /note="His-tag"
     CDS             109..780
                     /vntifkey="4"
                     /label=gst
                     /note="codes for Gst"
     misc_feature    790..804
                     /vntifkey="21"
                     /label=EK
                     /note="enterokinase recognition site"
BASE COUNT     1460 a      1562 c      1625 g      1454 t 
ORIGIN
        1 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa 
       61 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gtcccctata 
      121 ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt 
      181 gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa 
      241 aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa 
      301 ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt 
      361 tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac 
      421 ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc 
      481 aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat 
      541 ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac 
      601 atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa 
      661 gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag 
      721 ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatcggatct ggttccgcgt 
      781 ccatggtcgg acgatgacga taaggatcat atgtcgtact accatcacca tcaccatcac 
      841 ttgactgtat cgccggaatt cgggcccgga tccctgcagc caagctaatt ccggcttgaa 
      901 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 
      961 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     1021 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     1081 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     1141 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     1201 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     1261 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     1321 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 
     1381 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     1441 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     1501 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     1561 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     1621 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 
     1681 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     1741 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     1801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     1861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     1921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     1981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     2041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     2101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     2161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     2221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 
     2281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     2341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     2401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     2461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     2521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     2581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     2641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     2701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     2761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     2821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     2881 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 
     2941 gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 
     3001 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg 
     3061 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 
     3121 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 
     3181 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc 
     3241 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata 
     3301 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg 
     3361 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg 
     3421 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta 
     3481 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca 
     3541 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg 
     3601 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt 
     3661 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt 
     3721 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac 
     3781 gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc 
     3841 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca 
     3901 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg 
     3961 aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg 
     4021 cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc 
     4081 tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg 
     4141 taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca 
     4201 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga 
     4261 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca 
     4321 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg 
     4381 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc 
     4441 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga 
     4501 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc 
     4561 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat 
     4621 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 
     4681 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 
     4741 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc 
     4801 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa 
     4861 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat 
     4921 cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg 
     4981 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc 
     5041 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc 
     5101 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag 
     5161 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc 
     5221 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg 
     5281 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca 
     5341 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg 
     5401 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg 
     5461 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg 
     5521 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc 
     5581 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc 
     5641 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca 
     5701 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct 
     5761 tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc 
     5821 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc 
     5881 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc 
     5941 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg 
     6001 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc 
     6061 gccggtgatg ccggccacga tgcgtccggc gtagaggatc g 
//
