| This is a preprint version of an article published in Methods in Enzymology*, Applications of Chimeric Genes and Hybrid Proteins, Part C, Protein-Protein Interactions and Genomics, eds John Abelson, Scott Emr, and Jeremy Thorner, vol 328, pp26-46, 2000
*the only definitive repository of the content that has been certified and accepted after peer review. Copyright and all rights therein are retained by Academic Press. This material may not be copied or reposted without explicit permission. Copyright 2000 Academic Press.
Interaction mating methods in two-hybrid systems
Mikhail G. Kolonin, Jinhui Zhong, and Russell L. Finley Jr.
Center for Molecular Medicine and Genetics
Wayne State University School of Medicine
Detroit, Michigan 48201
Paper in PDF format
OUTLINE
Introduction
Isolating new interactors from libraries
- Constructing and characterizing the bait strain
Preparing the pretransformed library and control strains
Screening for interactors by mating
Characterizing interactors
Testing interactions between small sets of proteins by cross-mating assay
Mapping networks of interacting proteins
Exploring the functions of individual protein interactions
Concluding remarks
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