Finley Lab
Center for Molecular Medicine and Genetics

| Finley Lab | IM Browser | DroID | Protocols/Reagents | People | Contact

WSU logo

Center for Molecular Medicine and Genetics
Wayne State University School of Medicine
540 E. Canfield
Detroit, MI 48201



High Throughput Cloning

Recombination Cloning in E. coli

Protocol for cloning approach described in Parrish et al., J. Proteome Research 3(3):582-6, 2004.; DOI: 10.1021/pr0341134.

Protocols for the LexA/Brent lab version of the yeast  two-hybrid system.

Interaction mating methods in two-hybrid systems

Methods for library screening, interactive screening, and use of peptide aptamers.

Performing a hunt by mating
Our latest two-hybrid hunt protocol update, and the current method of choice in our lab.

Protocol update paper from April 1997
Still a useful supplement to the mating version.

The original two-hybrid interactor hunt protocol
Still a useful place to look for background info, media recipes, and references.

Yeast transformation - LiOAc method

Strains and Vectors

Yeast and E. coli strains

Vector Maps and sequences

C. jejuni GST-His-tagged expression clone set


Finley Lab libraries


Yeast two-hybrid web sites


  Finley Lab | projects | CMMG home | bookmarks | LabFMdb|