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Finley and Brent
Table 1. Activating and non-activating baits.
Bait |
Activation |
Color on X-Gal* |
Plasmid name |
Reference |
LexA-Gal4 |
strong |
blue
|
pSH17-4 |
S. Hanes, unpublished; Golemis and Brent, 1992
|
LexA-Fos |
strong |
blue
|
VR1001 |
Lech et al., 1988; Golemis and Brent, 1992
|
LexA-Myc |
strong |
blue
|
VR1004 |
Lech et al., 1988; Golemis and Brent, 1992
|
LexA- Cdi2 |
moderate |
light blue
|
pRF202-Cdi2 |
Finley and Brent, 1994
|
LexA-HsCycC |
moderate |
light blue
|
pJG28-2 |
J. Gyuris and R.B., unpublished
|
LexA-DmErk |
moderate |
light blue
|
pEG202-ERK |
Zavitz, Zipursky, Finley, and Brent, unpublished
|
LexA-Mxi1 |
weak |
very light blue
|
pLexA-Mxi1 |
Zervos et al., 1993
|
LexA-NF2 (N) |
weak |
very light blue
|
pAR-NF2(N-term) |
A. Reymond and R.B, unpublished
|
LexA-DmCdi11 |
weak |
very light blue
|
pRF202-Cdi11 |
Finley and Brent, 1994
|
LexA-BcdÆC |
none |
white
|
pRFHM1 |
Gyuris et al., 1993
|
LexA-Max |
none |
white
|
pLexA-Max |
Zervos et al., 1993
|
LexA-HsCdc2 |
none |
white
|
pJG14-1 |
Gyuris et al., 1993
|
* As determined on typical fresh X-Gal galactose/raffinose plates
in a diploid strain containing pSH18-34 (S.Hanes, unpublished),
the sensitive LexAop-lacZ reporter plasmid (R. Finley,
unpublished data).
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