Finley Lab
Center for Molecular Medicine and Genetics

| Finley Lab | IM Browser | DroID | Protocols/Reagents | People | Contact












WSU logo

Center for Molecular Medicine and Genetics
Wayne State University School of Medicine
540 E. Canfield
Detroit, MI 48201

 
 


Vectors

The sequence files available here were assembled and annotated based on published plasmids and sequences available in Genbank. The .gb files are text files in the Genbank format. The pdf contains annotated sequences and the jpg is a map.

The size of each file is in parentheses following the file name.

pHZ5

HIS3 2 MAL62p LexA NLS. HIS3 2 yeast vector for regulated expression of N-terminal LexA fusions using the MAL62 promoter. Includes at the C-terminal end of LexA. This is the BD vector currently used by the Finley lab for most yeast two-hybrid studies. Finley et al., 2002, Gene 285, 49-57.
pHZ5.jpg (212kb) pHZ5.gb (20kb)  pHZ5.pdf(1.2mb)

pHZ5attR

HIS3 2 MAL62p LexA NLS attR. Destination vector version of pHZ5 for use with the GatewayTM (Invitrogen) in vitro cloning system. Finley et al., 2002, Gene 285, 49-57.
pHZ5attR.jpg(380kb) pHZ5attR.gb(24kb) pHZ5attR.pdf(380kb)

pLexA(202+PL)

HIS3 2 ADH1p LexA. This is the granddaddy of all LexA expression vectors. HIS3 2 with the ADH1 promoter driving expression of LexA. Most (possibly all) of the other LexA vectors shown here were derived from this common ancestor. Ruden et al., 1991, Nature 350, 250-252.
pLexA(202+PL).jpg (276kb) pLexA(202+PL).gb(20kb) pLexA(202+PL).pdf(1.2mb)

pEG202

HIS3 2 ADH1p LexA mcs. The most common plasmid used in the LexA two-hybrid system. Similar to pLexA(202+PL) but with a better multiple cloning site downstream of LexA. The sequence file was derived from the version marketed by Clontech and from Genbank Accession Number U89960. Estojak et al., 1995, Mol. Cell. Biol. 15, 5820-5829.
pEG202.jpg(308kb) [U89960 (GenBank site) ] pEG202.pdf(1.2mb)

pNLex [also known as pNLex(NLS)]

HIS3 2 ADH1p LexA NLS. Derived from pLexA(202+PL). Contains an SV40 nuclear localization signal (NLS) coding sequence downstream of LexA and upstream of the mcs.
pNLex(NLS).jpg(280kb) pNLex(NLS).gb(20kb) pNLex(NLS).pdf(1.2mb)

pNLexAattR

HIS3 2 ADH1p LexA NLS attR. Destination version of pNLex for use with the GatewayTM (Invitrogen) in vitro cloning system. Finley et al., 2002, Gene 285, 49-57.
pNLexAattR.jpg(372kb) pNLexAattR.gb(24kb) pNLexAattR.pdf(1.4mb)

pJZ4

TRP1 2 GAL1p NLS-B42AD-HAtag mcs CYC1t. For regulated expression of AD fusions from the GAL1 promoter. Similar to pJG4-5 except with an F1 origin and the CYC1 terminator instead of ADH1t to reduce possible recombination with the BD vector. This is the AD vector currently used by the Finley lab for most yeast two-hybrid studies.
pJZ4.jpg(332kb) pJZ4.gb(16kb) pJZ4.pdf(952kb)

pJZ4attR

TRP1 2µ GAL1p NLS-B42AD-HAtag mcs CYC1t. Gateway destination vector version of pJZ4.

pJZ4attR.pdf (map) (65kb) pJZ4attR.g (sequence) (18 kb)

pJG4-5

TRP1 2 GAL1p NLS-B42AD-HAtag mcs ADH1t. This is the original AD vector for the Brent lab LexA two-hybrid system. The sequence file was derived from the version marketed by Clontech and from Genbank Accession Number U89961. Gyuris et al., 1993, Cell 75, 791-803.
pJG4-5.jpg(344kb) [ U89961 (GenBank site) ] pJG4-5.pdf(920kb)

pHZ5attB-DmCycEI

Described in Zhong et al. pHZ5 derivative for expressing LexA fused to Drosophila cyclin E type I.

pNLexAattB-DmCycEI

Described in Zhong et al. pHZ5 derivative for expressing LexA fused to Drosophila cyclin E type I.
pNLexAattB-DmCycEI.jpg(348kb) pNLexAattB-DmCycEI.gb(20kb) pNLexAattB-DmCycEI.pdf (1.7mb)

Destination vectors from Stanyon et al.

Yeast expression vectors for use with the GatewayTM (Invitrogen) in vitro cloning system. Stanyon et al., 2003, Biotechniques 35, 520-536.
StanyonVect.jpg (252kb)

pTLJ03

Described in Parrish et al., 2004.  E. coli protein expression vector containing 5RT1 and 3RT1 recombination tags to facilitate ORF cloning.  Inducible expression from a T7 promoter generates N-terminal GST-6HIS fusions to proteins of interest.
pTLJ03.jpg (244kb) pTLJ03.gb (12kb)  pTLJ03.pdf(172kb)


More from Gene 285, 49-57 (2002)

pHZ5-RT

pHZ5 lacking the NLS after LexA, but with 5' and 3' Recombination Tags.

pHZ5-NRT

pHZ5 with 5' and 3' Recombination tags

Unpublished

pRF4-6o

TRP1, 2, yeast vector for expressing HA-epitope-tagged (N-terminal) proteins in yeast from the GAL1 promoter. Unpublished.
pRF4-6o.jpg (196kb) pRF4-6o.gb (80kb)  pRF4-6o.pdf(1.1mb)


 
  Finley Lab | projects | CMMG home | bookmarks | LabFMdb|