Finley Lab
Center for Molecular Medicine and Genetics

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Quick tutorial
System Requirements
Description of interface
Input data
Connection to a database
Query forms to add/filter/color a graph
Add Interactions
Expand nodes
Filter a graph
Color a graph
Layout a graph
Node and edge information
Save options
Deployment
Color graph
By gene attributes
By interaction attributes
By table name
By table count
Edit color schema
Color a set of nodes/edges

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Center for Molecular Medicine and Genetics
Wayne State University School of Medicine
540 E. Canfield
Detroit, MI 48201

IM Browser Manual

Add interactions to a graph by gene/protein attributes


First we will look at a simple example. Then we will give more details about the search by gene/protein attributes.

Example: Build a graph of interactions for genes of cyclin family (gene name starts with "cyc").

From the main menu select 'Edit'->'Add Gene/Protein'.

Alternatively, from the main menu select 'File'->'New graph'.

Select button “Search for Gene/Pprotein in look-up table(s)”.



A new dialog box will appear.

In the pull-down menu select “Symbol”.

In the text area enter 'cyc*'.

Note: Be sure to use the wild card '*' to find all of the cyc genes.



IM Browser will display a list of genes that satisfy your search criteria.

Select the genes of interest either by clicking on "Select All" or by clicking on check boxes.

Click “OK”.



Now we are back to the query form.

The IDs of the selected genes were added to the text area.

You can conduct additional searches and the new IDs will be added to the text area.

Select the interaction tables to search.

Click OK.



The resulting graph consists of the interactions of cyc family genes found in tables "Hybrigenics YTH" and "Finley YTH".



The search by gene/protein attributes is not case sensitive. Queries can be built by exact search term or with wild cards “?” and “*” for a partial terms. In addition to searches on individual attributes, an “Any Field” choice allows all of the fields to be searched simultaneously with the search term entered.

A single gene may have several values for the same attribute; for example, a gene might have several synonyms or several molecular functions. In some databases attributes with multiple values are decomposed into separate tables. In others all values of the same attribute are written in one field, each value delimited by a symbol. IM Browser works with both data organizations. In the first case, when each attribute value stored in a separate row, the user enters the exact value; and in the second case, when several values are stored in one field, user embeds the value into “*”.

For the Drosophila database we included an option for the users to connect directly to Flybase and search its extensive, frequently updated gene and protein information. When deciding whether to search look-up tables or the Flybase database, users should consider the following: searching in look-up tables is much faster than in Flybase database, however look-up tables might contain out-of date information while Flybase is always up to date.


If the user knows Ids of genes/proteins, one can simply type them in the text area.




If the user types '*' in the text area, then all the interactions from the selected tables will be added to the graph.


If the user has a file with the list of gene/protein IDs, one can load the Ids through the button "Load gene/protein IDs from a file".

The Ids in the file can be either comma or line break delimited.